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Strain
B6.Cg(129S4)-Ppardtm1Rev Tg(Nes-cre)1Kln

Summary Data Summary
Official Name B6.Cg(129S4)-Ppardtm1Rev Tg(Nes-cre)1Kln
Common Name B6.Cg-Ppardtm1Rev Tg(Nes-cre)1Kln
Description C57BL/6J Neuronal PPAR Delta KO

Ppard knockin: Exon 4, which encodes the amino terminal
portion of the DNA binding domain was left flanked by single
loxP sites after a downstream neo cassette was excised via
the expression of cre recombinase in vitro.

Tg(Nes-cre): This transgene expresses Cre recombinase with a
human growth hormone polyadenylation signal under the
control of a rat nestin (Nes) promoter and the nervous
system-specific enhancer present in the second intron. This
promoter expresses Cre recombinase in neuronal and glial
cell precursors. Cre recombinase activity is present in
nervous tissue by embryonic day 11. The transgene was
localized to chromosome 12 using FISH with a rat nestin
promoter probe (J:180046). The insertion caused a 17 bp
deletion.
Development Status Phenotyping complete
Creation Method knockout
Background C57BL/6J
TypeCount
Investigators 1
Genomics - Modifications 1
Genomics - Transgenes 1
Experiments 1
Publications 1


Investigators
NameInstitution
Kevin NiswenderVanderbilt University


Genomic Information
GeneAllele 1Allele 2Protocol
Ppardknockin (lox)knockin (lox)Not Specified
EnhancerPromoterGeneCopies
Not SpecifiedNesCRENot Specified






PublicationAltmetricsSubmitted ByPubMed IDStatus
Neuron-specific deletion of peroxisome proliferator-activated receptor delta (PPARd) in mice leads to increased susceptibility to diet-induced obesity.
Kocalis HE, Turney MK, Printz RL, Laryea GN, Muglia LJ, Davies SS, Stanwood GD, McGuinness OP, Niswender KD
PLoS ONE, 2012 (7), e42981
22916190
Published

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