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Strain
B6.129S-Insrtm1Khn Igf1tm1Dlr

Summary Data Summary
Official Name B6.129S-Insrtm1Khn Igf1tm1Dlr
Common Name B6.129S-Insrlox/lox Igf1lox/lox
Description B6 and 129/Sv background with the Insr (insulin receptor)
and Igf1 (insulin like growth factor 1) genes were floxed.

Insr flox: Exon 4 was left flanked by single loxP sites
after a downstream floxed neo cassette was removed via in
vitro cre mediated recombination. Deletion of the resultant
floxed fragment will result in a frameshift mutation that
introduces a stop codon. Translation, if it were to occur,
would putatively produce a truncated peptide consisting of
308 amino terminal residues and lacking the high affinity
binding site, transmembrane domain, and kinase domain.

Igf1 flox: A neomycin cassette and loxP sites were inserted
flanking exon 4. The introduction of these mutations had no
effect on the normal function of the gene.
Development Status Phenotyping ongoing
Creation Method knockin
Breeding Type intercross
TypeCount
Investigators 1
Genomics - Modifications 2
Experiments 1
Publications 1


Investigators
NameInstitution
Jeremie BoucherHarvard Medical School


Genomic Information
GeneAllele 1Allele 2Protocol
Insrknockin (lox)knockin (lox)Not Specified
Igf1knockin (lox)knockin (lox)Not Specified






PublicationAltmetricsSubmitted ByPubMed IDStatus
Impaired thermogenesis and adipose tissue development in mice with fat-specific disruption of insulin and IGF-1 signalling.
Boucher J, Mori MA, Lee KY, Smyth G, Liew CW, Macotela Y, Rourk M, Bluher M, Russell SJ, Kahn CR
Nature communications, 2012 (3), 902
22692545
Published

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