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Strain
C57BL/6-Plgtm1Jld Fgatm1Jld/+

Summary Data Summary
Official Name C57BL/6-Plgtm1Jld Fgatm1Jld/+
Common Name C57BL/6-Plgtm1 Fgatm1/+
Description C56BL/6J mice with homozygous knockout of Plasminogen and
heterozygous KO/Wildtype for the Fibrinogen alpha gene.

Plg KO: A PGK-HPRT minigene replaces proximal promoter
sequences and the first two exons of the Plg gene. Northern
analysis and enzymatic activity assays were used to
demonstrate the lack of a transcript and functional protein,
respectively, in homozygous mutant animals.

Fga KO: An HPRT minigene cassette was inserted into exon 1
in sequences corresponding to the the signal peptide
sequence of the Fga gene. Transcripts from this allele were
not detected in total liver RNA derived from homozygous mice
and an in vitro clotting assay demonstrated that no
functional fibrinogen protein was present in plasma derived
from homozygous mice.
Development Status Phenotyping ongoing
Creation Method knockout
Background C57BL/6J
Breeding Type intercross
Breeding Generations 11
TypeCount
Investigators 1
Genomics - Modifications 2
Experiments 1
Publications 1


Investigators
NameInstitution
Jonathan SchoeneckerVanderbilt University


Genomic Information
GeneAllele 1Allele 2Protocol
PlgknockoutknockoutNot Specified
FgaknockoutwildNot Specified


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