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Strain
C57BL/6-Gt(ROSA)26Sortm1(HBEGF)Awai Tg(Gh1-cre)bKnmn

Summary Data Summary
Official Name C57BL/6-Gt(ROSA)26Sortm1(HBEGF)Awai Tg(Gh1-cre)bKnmn
Common Name C57BL/6-Tg(Gh1-cre)bKnmn iDTR
Description AOiGHD mice are generated by breeding rGHp-Cre mice (Cre) to
inducible diphtheria toxin receptor mice (iDTR).
Somatotropes of Cre+,iDTR+ express DTR. In the absence of
the toxin pituitary/somatotrope morphology and function is
normal, compared to WT or Cre–,iDTR+ controls. When adult
Cre+,iDTR+ mice are treated with DT, somatotropes are
selectively destroyed and circulating GH/Igf1 reduced
(AOiGHD), compared to DT-treated Cre¬-,iDTR+ mice
(GH-INTACT). AOiGHD fed standard chow (17% kcal from fat) do
not differ in body weight, but have larger fat depots,
compared to GH-INTACT. AOiGHD show improved response to ITT
and have reduced fed insulin and glucose, compared to
GH-INTACT, while response to GTT is unchanged. To mimic a
more “western” diet, Cre+,iDTR+ and Cre-,iDTR+ mice (3
months) were treated with DT (osmotic pumps, 6ng/h for 7d)
and fed either a low fat (LF,10%) or high fat (HF,45%) diet.
At 8 months the respiratory quotient (VCO2/VO2, as
determined by indirect calorimetry) of AOiGHD was
significantly elevated compared to GH-INTACT and this
difference was more pronounced during the absorptive state
with LF feeding. These results indicate AOiGHD utilize more
carbohydrates for cellular metabolism, consistent with
improved insulin sensitivity. The response to GTT at 9
months did not differ between LF-fed AOiGHD and GH-INTACT,
however glucose clearance in HF-fed AOiGHD was impaired and
fasting insulin reduced, relative to HF-fed GH-INTACT. Using
Igf1 as a marker of GHD, fasting Igf1 was positively
correlated with insulin within diet. Taken together these
results suggest that lowering adult GH/Igf1 improves insulin
sensitivity, but GH/Igf1 is required to maintain beta-cell
function.
Development Status Phenotyping ongoing
Creation Method transgenic
Background C57BL/6J
Breeding Type intercross
TypeCount
Investigators 1
Genomics - Modifications 1
Genomics - Transgenes 1
Experiments 3
Publications 1


Investigators
NameInstitution
Rhonda KinemanUniversity of Illinois at Chicago


Genomic Information
GeneAllele 1Allele 2Protocol
Gt(ROSA)26Sorknockin (lox)knockin (lox)Not Specified
EnhancerPromoterGeneCopies
Not SpecifiedGhCRENot Specified






PublicationAltmetricsSubmitted ByPubMed IDStatus
Differential impact of selective GH deficiency and endogenous GH excess on insulin-mediated actions in muscle and liver of male mice.
Cordoba-Chacon J, Gahete MD, McGuinness OP, Kineman RD
American journal of physiology. Endocrinology and metabolism, 2014 (307), E928 - E934
25269484
Published

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