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Strain
B6.129S2-Thbs1tm1Hyn/J

Summary Data Summary
Official Name B6.129S2-Thbs1tm1Hyn/J
Common Name B6.129S2-Thbs1tm1Hyn/J
Description A neomycin selection cassette replaced exon 2, intron 3, and
exon 3. An RNase protection assay demonstrated that no
detectable transcript was present in multiple tissues of
homozygous animals. Western analysis confirmed the absence
of the encoded protein.
Development Status Phenotyping ongoing
Creation Method knockout
Phenotype Description Mice homozygous for this targeted mutation are viable and
fertile, with an approximate 20% decrease in embryo/neonate
viability and a mild and variable lordotic curvature of the
spine apparent from birth. Homozygous mice have an abnormal,
but no full length transcript in multiple tissues. Western
analysis confirmed the absence of the protein in platelets.
Homozygotes exhibit an increase in the number of circulating
white blood cells. During the first four to ten weeks of
life, homozygotes exhibit patches of acute and organizing
pneumonia. At later time points, there is considerable
hyperplasia of the various epithelial cell lineages. Mutant
mice also have an increased number of retinal endothelial
cells and inappropriate remodeling and maturation of retinal
vasculature following injury. On the FVB/N background,
spontaneous tumor growth and vasculature are significantly
increased compared to wildtype. Mutant mice may be useful in
studies of inflammatory responses in the lungs, eye, and
skin, angiogenesis and vascular pathophysiology, cancer,
chemotherapy, apoptosis, and cell differentiation and
migration.
TypeCount
Investigators 1
Genomics - Modifications 1
Experiments 4
Publications 1


Investigators
NameInstitution
Tae-Hwa ChunUniversity of Michigan-Ann Arbor


Genomic Information
GeneAllele 1Allele 2Protocol
Thbs1knockoutknockoutNot Specified






PublicationAltmetricsSubmitted ByPubMed IDStatus
Thrombospondin 1 mediates high-fat diet-induced muscle fibrosis and insulin resistance in male mice.
Inoue M, Jiang Y, Barnes RH, Tokunaga M, Martinez-Santibañez G, Geletka L, Lumeng CN, Buchner DA, Chun TH
Endocrinology, 2013 (154), 4548 - 4559
24140711
Published

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