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Experiment

Hyperinsulinemic Euglycemic Clamp Order#2176 2/8/11
Summary Data Summary
Investigator Chun, Tae-Hwa
Description Hyperinsulinemic-euglycemic clamp: Insulin is continuously infused at a constant
rate during 155min (see data for infusion rate), and glucose is infused at a
variable rate to maintain euglycemia. 3H-glucose is infused to assess glucose
fluxes. At 120min, a bolus of 14C-2-deoxyglucose is given to assess
tissue-specific glucose uptake. Mice are sacrificed at 155min.

Mouse were fasted for 5h prior to experiment.
Water: regular tap water, ad libitum.

Groups: WT and KO

Purpose of the experiment: This experiment will determine whether the groups of
mice have different insulin sensitivity, and will determine sites of insulin
resistance.

Applicable Research Areas: Metabolism, Insulin resistance.
Status Completed
Public Release 6/6/2015
Animal Age Measured In: day(s) post-natal (d)
Flags has-data-flagExperiment Description
Data Analysis
TypeCount
Animals23
Experimental Conditions5
Catalog Items2
Curation Info (# flags)1
Phenotype Assays33
Phenotype Measurements7782
Histology Images0
Publications0
Phenotypes7


Animals

Strain NameCommon NameFemalesMalesUnknown
C57BL/6J
0
11
0
B6.129S2-Thbs1tm1Hyn/J
0
12
0


Experimental Conditions
NameUnits
Experimental Groupone of [Control, Control-Het, ...]
Glucose Infusion Rate (mg/kg/min)milligrams per kilogram per minute (mg/kg/min)
Insulin Infusion Rate - milliUnitsmilliUnits per kilogram per minute (mU/kg/min)
Mouse Dietone of [3mAIN76(HF0.18), 3mAIN76(HF0.18)early, ...]
Time Sample Takenminute (min)



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