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Strain
B6(Cg)-Snord116tm1.1Uta/J

Summary Data Summary
Official Name B6(Cg)-Snord116tm1.1Uta/J
Common Name Snord116 -/+
Description Two individual targeting vectors were used to place a loxP
site (and an Frt1-flanked PGK-neo cassette) upstream, and a
loxP site (and an Frt5-flanked puromycin resistance/TK
cassette) downstream of the Snord116 cluster. The upstream
targeting vector was transfected into C57BL/6-derived
Bruce-4 embryonic stem (ES) cells, and correctly targeted ES
cells were next transfected with the downstream targeting
vector. Doubly targeted ES cells were then transiently
transfected with a cre expressing plasmid. The resulting
1-loxP ES cells (with the Snord116 cluster deleted and a
single loxP site remaining) were injected into recipient
blastocysts.
Development Status Not specified/Other
Creation Method knockout
TypeCount
Investigators 1
Genomics - Modifications 1
Child Strains 2
Experiments 2


Investigators
NameInstitution
Dag YasuiUniversity of California Davis


Genomic Information
GeneAllele 1Allele 2Protocol
Snord116knockoutwildNot Specified


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