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Strain
C57BL/6-Atp6ap2tm1.1Aich Tg(Nefh-cre)12Kul

Summary Data Summary
Official Name C57BL/6-Atp6ap2tm1.1Aich Tg(Nefh-cre)12Kul
Common Name Nefh-PRRKO
Description Atp6ap2: A loxP site was inserted upstream of exon 2 and was
followed by an FRT flanked neomycin and a second loxP site.
A third loxP site was placed immedicately downstream of exon
2. Properly targeted mice were bred to mice expressing flp
recombinase in order to remove the neomycin selection
cassette and leave exon 2 surrounded by loxP sites.

Nefh-cre: An 8.5 kb promoter fragment of mouse Nefh,
containing the 5' UTR and 40 bp of non-coding sequence of
exon 1 ending before the start codon, was adjoined to
sequence encoding cre recombinase with an SV40 nuclear
localization signal. An SV40 polyadenylation signal was also
included in the construct. Expression of cre recombinase is
driven in neurons of the brain and spinal cord during late
development (around E18.5). Line 12 is best described and is
representative of the other lines.
Development Status Phenotyping ongoing
Creation Method knockout
Breeding Type intercross
TypeCount
Investigators 1
Genomics - Modifications 1
Genomics - Transgenes 1
Experiments 3


Investigators
NameInstitution
Caleb WorkerColorado State University


Genomic Information
GeneAllele 1Allele 2Protocol
Atp6ap2knockin (lox)knockin (lox)Not Specified
EnhancerPromoterGeneCopies
Not SpecifiedNefhCRENot Specified


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