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Strain
C57BL/6J-Irf3tm1Ttg

Summary Data Summary
Official Name C57BL/6J-Irf3tm1Ttg
Common Name C57BL/6J-Irf3-/-
Description Global knockout of interferon regulatory factor 3 (Irf3).

A pgk-neo cassette replaced a 2.2Kb region that contains a
putative transcription initiation site and the coding exon
for the N-terminal part of the DNA binding domain via
homologous recombination. Immunoblot analysis of MEFs
confirmed loss of protein. A subsequent publication
demonstrated the neo cassette also replaced the first two
exons of a second locus located on the opposite strand. This
resulted in the loss of the transcription and translational
start sites of the second locus. mRNA analysis of numerous
tissues from homozygous mice demonstrated transcript levels
were 50% to 100% lower than endogenous levels.
Development Status Phenotyping ongoing
Creation Method knockout
Background C57BL/6J
Phenotype Description Homozygous null mice are more susceptible to viral infection
and exhibit lower serum interferon levels in response to
viral infection.
TypeCount
Investigators 1
Genomics - Modifications 1
Experiments 1
Publications 1


Investigators
NameInstitution
Evan RosenBeth Israel Deaconess Medical


Genomic Information
GeneAllele 1Allele 2Protocol
Irf3knockoutknockoutNot Specified






PublicationAltmetricsSubmitted ByPubMed IDStatus
IRF3 promotes adipose inflammation and insulin resistance and represses browning.
Kumari M, Wang X, Lantier L, Lyubetskaya A, Eguchi J, Kang S, Tenen D, Roh HC, Kong X, Kazak L, Ahmad R, Rosen ED
The Journal of clinical investigation, 2016 (126), 2839 - 54
Submitted Externally
27400129
Published

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