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Strain
B6.129(SJL)-Nrp1tm2Ddg Tg(Cdh5-cre/ERT2)1Rha

Summary Data Summary
Official Name B6.129(SJL)-Nrp1tm2Ddg Tg(Cdh5-cre/ERT2)1Rha
Common Name B6.129(SJL)-Nrp1tm2Ddg Tg(Cdh5-cre/ERT2)1Rha
Description Exon 2 of Nrp1 was replaced with a mutated exon 2 encoding a
7 amino acid substitution in three regions of the
amino-terminal CUB domain and a Pgk-neo cassette flanked by
loxP sites was inserted within the intron upstream of exon
2. The Pgk-neo cassette was removed by mating with mice
expressing germline Cre-recombinase. Mutation of this site
was shown to abolish Sema3 family binding but not VEGF
binding. Immunohistochemistry showed that homozygotes
express normal levels of protein.

The tamoxifen-inducible cre/ERT2 sequence was inserted into
a PAC (P1 artificial chromosome) containing the VE-cadherin
(Cdh5) gene to generate the transgene construct.
Development Status Phenotyping ongoing
Creation Method knockout
Breeding Type intercross
TypeCount
Investigators 1
Genomics - Modifications 1
Genomics - Transgenes 1
Experiments 2


Investigators
NameInstitution
Emma GordonYale University


Genomic Information
GeneAllele 1Allele 2Protocol
Nrp1knockin (lox)knockin (lox)Not Specified
EnhancerPromoterGeneCopies
Not SpecifiedCdh5CRENot Specified


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