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Experiment

Nrp1 CLAMS

Summary

Data Summary

Investigator	Gordon, Emma
Description	The interaction of growth factor VEGF-B with the endothelial receptor VEGF receptor 1 (VEGFR1, also known as FLT1) and the co-receptor neuropilin 1 (NRP1) induces transcriptional upregulation of the vascular-specific fatty acid transport protein 3 (Fatp3, also known as Slc27a3) and the more ubiquitously expressed Fatp4 (also known as Slc27a4). Mice lacking VEGF-B are healthy and fertile, but exhibit decreased fatty-acid uptake and lipid deposition in muscles. Instead, lipids are shunted to the white adipose tissue, and therefore Vegfb mice gain more weight (Hagberg et al., 2010, Nature). Conversely, cardiac overexpression of VEGF-B causes ceramide accumulation in the heart, and ultimately leads to mitochondrial dysfunction. Recently it was described that the vascular endothelium can function as an efficient barrier to excess muscle lipid uptake even under conditions of severe obesity and type 2 diabetes, and that this barrier can be maintained by inhibition of VEGF-B signaling (Hagberg et al., 2012, Nature). Given VEGF-B signals through endothelial receptor NRP1, we propose that targeting this receptor in endothelial cells will provide mice with resistance to type 2 diabetes.
Status	Completed
Public Release	7/1/2018
Animal Age	Measured In: week(s) post-natal (w)
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Data Analysis

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Type	Count
Animals	16
Experimental Conditions	2
Catalog Items	2
Curation Info (# flags)	3
Phenotype Assays	15
Phenotype Measurements	240
Histology Images	0
Publications	0
Phenotypes	6

Animals

Strain Name	Common Name	Females	Males	Unknown
C57BL/6	C57BL/6	0	8	0
B6.129(SJL)-Nrp1^tm2Ddg Tg(Cdh5-cre/ERT2)1Rha	B6.129(SJL)-Nrp1^tm2Ddg Tg(Cdh5-cre/ERT2)1Rha	0	8	0

Animals/Strains

Experimental Factors

	Categorical Values
	Name / Abbreviation	Description
	Experimental Factor: Experimental Group
	Control	This animal belongs to the control group for the experiment.
	Experiment	This animal belongs to experimental group that is homozygous for gene manipulations.
	Experimental Factor: Mouse Diet
	Research Diets - D12492	Rodent Diet with 34% Fat, 60% kcal from Fat

Experimental Factor (Units)
Experimental Group
Mouse Diet

Experimental Factor Values

Phenotype Assays Add / Edit

Name	Abbreviation
body mass	W rodent
water intake	water intake
energy expenditure	energy expenditure
oxygen consumption (Light Period)	VO2-L
fat body mass	fat body mass
lean body mass	lean body mass
respiratory exchange ratio (Light Period)	RER-L
oxygen consumption (Dark Period)	VO2-D
carbon dioxide production Light Period	VCO2-L
carbon dioxide production Dark Period	VCO2-D
respiratory exchange ratio (Dark Period)	RER-D
carbon dioxide production	VCO2
oxygen consumption	VO2
respiratory exchange ratio	RQ
Activity 24h (total)	Activity 24h (total)

No documents found.

Mammalian Phenotype (MP) Terms

MPTermID	MPTermName	Definition	Other Results
MP:0001262	decreased body weight	lower than normal average weight [PMID:10709991]	MMPC Search Results
MP:0004889	increased energy expenditure	increase in the number of calories used per unit time or decrease in weight gain for a given amount of food eaten [MGI:smb]	MMPC Search Results
MP:0005289	increased oxygen consumption	greater than the normal rate at which oxygen enters the blood from alveolar gas; this is equal in the steady state to the consumption of oxygen by tissue metabolism throughout the body [MESH:G06.535.723]	MMPC Search Results
MP:0008963	increased carbon dioxide production	increase in the rate at which carbon dioxide leaves the blood and enters the alveolar gas [MGI:csmith]	MMPC Search Results
MP:0010025	decreased total body fat amount	less than the normal total amount of connective tissue composed of fat cells within the entire body [MGI:csmith]	MMPC Search Results
MP:0014143	decreased body fat mass	decreased physical bulk or volume of fat in the whole body [MGI:csmith]	MMPC Search Results

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