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Strain
B6.129S7-Ldlrtm1Her Tg(APOB)1102Sgy

Summary Data Summary
Official Name B6.129S7-Ldlrtm1Her Tg(APOB)1102Sgy
Common Name B6.129S7-Ldlrtm1Her Tg(APOB)1102Sgy
Description Ldlr KO: Insertion of a neomycin resistance cassette into
exon 4. The authors predict that the targeted allele would
encode a truncated non-functional protein that will not bind
LDL, and that lacks a membrane spanning segment. Immunoblot
analysis of liver membranes detected a truncated protein in
homozygous mutant animals.

Tg(APOB): The transgene contains a 79.5 kb P1 insert with
the entire human APOB gene. RNA slot blot analysis detected
transgene expression in the liver, with very small amounts
expressed in the small intestine and heart. Western blot
analysis of plasma from transgenic animals showed human
apo-B100 as the predominant species of transgene product.
Development Status Phenotyping ongoing
Creation Method transgenic
Breeding Type intercross
TypeCount
Genomics - Modifications 1
Genomics - Transgenes 1
Experiments 2


Genomic Information
GeneAllele 1Allele 2Protocol
LdlrknockoutknockoutNot Specified
EnhancerPromoterGeneCopies
Not SpecifiedNot SpecifiedAPOBNot Specified


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