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Strain

129S/SvEv-Tg(Alb1-Ren)2Unc/CofJ

Summary

Data Summary

Official Name	129S/SvEv-Tg(Alb1-Ren)2Unc/CofJ
Common Name	RenTgMK
Description	A single copy of a transgene consisting of a liver specific albumin promoter, a 20 bp insert in the 5' UTR to increase length, a synthetic mouse renin cDNA, and a rabbit beta-globin 3' UTR was inserted into the apolipoprotein locus between the Apoa1 and Apoc3 genes. Mutations Made By Dr. Oliver Smithies, University of North Carolina
Development Status	Phenotyping ongoing
Creation Method	transgenic
Breeding Type	intercross
Phenotype Description	Mice heterozygous for the RenTgMK transgene are viable and fertile. The RenTgMK transgene contains a liver-specific albumin promoter/enhancer controlling the expression of a synthetic renin gene (engineered to allow efficient cleavage and secretion of the prorenin transgene product by the liver). This, and because the transgene was targeted to the apolipoprotein locus on Chromosome 9, results in active renin secretion from the liver independently of renal or other homeostatic cardiovascular control mechanisms (i.e. genetically clamped). RenTgMK heterozygotes have high ectopic levels of active mouse renin in the liver and elevated plasma levels of prorenin and active renin. Heterozygous mice display significantly elevated blood pressure, enhanced thirst, high urine output, proteinuria, and kidney damage. Because active renin overexpression results in increased circulating levels of angiotensin II (Ang II), heterozygotes also exhibit cardiac hypertrophy and 50% male mortality between 6-8 months of age. These RenTgMK mice may be useful in studying the effects of genetically clamped renin (and angiotensin II) on hypertension, diabetic nephropathy, albuninurea, nephrosclerosis, and other aspects of the renin?angiotensin system (RAS).

Type	Count
Investigators	1
Genomics - Transgenes	1
Experiments	1

Investigators

Name	Institution
Naima Moustaid-Moussa	University of Tennessee

Genomic Information

Enhancer	Promoter	Gene	Copies
Not Specified	Alb1	Ren1	Not Specified

OrderID	Order	Investigator	Experiment	Status	Measurements
1025	1025	Naima Moustaid-Moussa	Hyperinsulinemic-Euglycemic Clamp Order #1025 12/3/07	Completed	1379

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