mmpc-logo mmpc-logo
twitter-logo    bluesky-logo
| Create Account | login
Strain
B6.Cg-Arg1tm1Pmu Lyz2tm1(cre)Ifo

Summary Data Summary
Official Name B6.Cg-Arg1tm1Pmu Lyz2tm1(cre)Ifo
Common Name LysM-Cre;Arg1fl/fl
Description A loxP site was inserted upstream of exon 7 and a
loxP-flanked neomycin selection cassette was inserted
downstream of exon 8. Transient expression of cre
recombinase removed the neomycin cassette, leaving exons 7
and 8 floxed. These exons encode in addition to the
oligomerization domain, two highly conserved aspartic acid
residues that are essential to the enzymatic activity of the
gene product. Correct targeting was confirmed by genomic
PCR.

An NLS-cre gene was inserted into the endogenous ATG start
site of the gene. An frt-flanked neomycin cassette was
removed in ES cells via Flp-mediated recombination prior to
production of chimeric mice.
Development Status Phenotyping ongoing
Creation Method knockout
TypeCount
Investigators 1
Genomics - Modifications 2
Experiments 1


Investigators
NameInstitution
Sarah HuenYale University


Genomic Information
GeneAllele 1Allele 2Protocol
Arg1knockoutknockoutNot Specified
Lyz2knockin (Cre)wildNot Specified


Menu

Home
Contact
About MMPC
Animal Husbandry
Tests Data
Search Data
Analysis
Clients
MMPC Centers

Newsletter

Interested in receiving MMPC News?
twitter-logo Mouse Phenotyping
@NationalMMPC



2017 National MMPC. All Rights Reserved.