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A 0.8 kb genomic region between exons 1 and 2 was replaced
with a neomycin resistance cassette via homologous
recombination resulting in a shift in the downstream reading
frame. Northern blot analysis of homozygous mutant embryos
did not detect wild-type transcript, but instead detected a
larger mutant transcript which contained the neo insert.
Western blot of fetal livers did not detect protein product
in homozygous mutants.
Genomics - Modifications
Academy for Practical Nursing & Health Occupations
Curation Flag Information
New comment to be added:
Energy Expenditure Order #1722 7/13/09
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Financial support for this work was provided by the NIDDK Mouse Metabolic Phenotyping Centers (National MMPC, RRID:SCR_008997,
) under the MICROMouse Program, grants DK076169.
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