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Strain
C57BL/6-Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo Tg(Cdh5-cre/ERT2)1Rha

Summary Data Summary
Official Name C57BL/6-Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo Tg(Cdh5-cre/ERT2)1Rha
Common Name Cdh5-CreERT2;mT/mG
Description The targeting vector was designed with a CMV
enhancer/chicken beta-actin core promoter (pCA) driving
expression of a loxP-flanked, N-terminal membrane-tagged,
optimized DsRed fluorescent protein variant (called
tandem-dimer-Tomato or tdTomato) sequence followed by a
polyadenylation signal. Immediately distal to the second
loxP site is an N-terminal membrane-tagged, enhanced green
fluorescent protein (EGFP) sequence itself followed by a
polyadenylation signal. An frt-flanked neo cassette was also
located distal to the expression vector. Red fluorescence is
detected in all tissues tested. When mice are bred to Cre
expressing mice, the floxed region is excised in
Cre-expressing tissue and this allows expression of the EGFP
cassette.

The tamoxifen-inducible cre/ERT2 sequence was inserted into
a PAC (P1 artificial chromosome) containing the VE-cadherin
(Cdh5) gene to generate the transgene construct.
Development Status Phenotyping ongoing
Creation Method transgenic
Breeding Type intercross
TypeCount
Investigators 1
Genomics - Modifications 1
Genomics - Transgenes 1
Experiments 2
Publications 1


Investigators
NameInstitution
Pei-Yu ChenYale University


Genomic Information
GeneAllele 1Allele 2Protocol
Gt(ROSA)26Sorknockin (lox)knockin (lox)Not Specified
EnhancerPromoterGeneCopies
Not SpecifiedCdh5CRENot Specified






PublicationAltmetricsSubmitted ByPubMed IDStatus
The docking protein FRS2a is a critical regulator of VEGF receptors signaling.
Chen PY, Qin L, Zhuang ZW, Tellides G, Lax I, Schlessinger J, Simons M
Proceedings of the National Academy of Sciences of the United States of America, 2014 (111), 5514 - 9
Submitted Externally
24706887
Published

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