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Strain
B6.129-Prdm16tm1.1Brsp Tg(Adipoq-cre)1Evdr

Summary Data Summary
Official Name B6.129-Prdm16tm1.1Brsp Tg(Adipoq-cre)1Evdr
Common Name Prdm16 FKO
Description Prdm16 lox: The targeting vector was designed to insert a
loxP site upstream of exon 9 followed by a frt-flanked
neomycin resistance (neo) cassette, and a second loxP site
downstream of exon 9.

Tg(Adipoq-cre): To generate the Adipoq-Cre BAC transgenic
mice, the 245 kb RP23-90G21 bacterial artificial chromosome
(BAC) containing at least five mouse genes was modified by
replacing the starting ATG and 222 bp of the adiponectin
gene with the starting ATG and coding sequence of a Cre
recombinase gene. Founder line 1 was analyzed. These mice
express Cre recombinase effectively in white adipose tissue
(WAT) and brown adipose tissue (BAT), but not in macrophages
(including adipose-tissue resident macrophages, alveolar
macrophages, or thioglycollate-stimulated peritoneal
macrophages). The donating investigator reports highly
efficient Cre recombinase activity, with no ectopic
expression.
Development Status Phenotyping ongoing
Creation Method knockout
TypeCount
Investigators 1
Genomics - Modifications 1
Genomics - Transgenes 1
Experiments 1
Publications 1


Investigators
NameInstitution
Bruce SpiegelmanHarvard University


Genomic Information
GeneAllele 1Allele 2Protocol
Prdm16knockin (lox)knockin (lox)Not Specified
EnhancerPromoterGeneCopies
Not SpecifiedAdipoqCRENot Specified






PublicationAltmetricsSubmitted ByPubMed IDStatus
Ablation of PRDM16 and beige adipose causes metabolic dysfunction and a subcutaneous to visceral fat switch.
Cohen P, Levy JD, Zhang Y, Frontini A, Kolodin DP, Svensson KJ, Lo JC, Zeng X, Ye L, Khandekar MJ, Wu J, Gunawardana SC, Banks AS, Camporez JP, Jurczak MJ, Kajimura S, Piston DW, Mathis D, Cinti S, Shulman GI, Seale P, Spiegelman BM
Cell, 2014 (156), 304 - 16
Submitted Externally
24439384
Published

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