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Experiment

PRDM16 fKO clamp

Summary

Data Summary

Investigator	Spiegelman, Bruce
Description	A clear relationship exists between visceral obesity and type 2 diabetes, whereas subcutaneous obesity is comparatively benign. Here we show that adipocyte-specific deletion of the coregulatory protein PRDM16 caused minimal effects on classical brown fat but markedly inhibited beige adipocyte function in subcutaneous fat following cold exposure or ß3-agonist treatment. These animals developed obesity on a high fat diet, with severe insulin resistance and hepatic steatosis. They also showed altered fat distribution with markedly increased subcutaneous adiposity. Subcutaneous adipose tissue in mutant mice acquired many key properties of visceral fat, including decreased thermogenic and increased inflammatory gene expression and increased macrophage accumulation. Transplantation of subcutaneous fat into mice with diet-induced obesity showed a loss of metabolic benefit when tissues were derived from PRDM16 mutant animals. These findings indicate that PRDM16 and beige adipocytes are required for the “browning” of white fat and the healthful effects of subcutaneous adipose tissue. Applicable research area(s): Diabetes, Obesity, Metabolism
Status	Completed
Public Release	7/15/2018
Data Collected?	Data will not be collected for this catalog item
Animal Age	Measured In: week(s) post-natal (w)
Flags

Data Analysis

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Type	Count
Animals	15
Experimental Conditions	1
Catalog Items	1
Curation Info (# flags)	3
Phenotype Assays	13
Phenotype Measurements	195
Histology Images	0
Publications	0
Phenotypes	4

Animals

Strain Name	Common Name	Females	Males	Unknown
C57BL/6	C57BL/6	0	8	0
B6.129-Prdm16^tm1.1Brsp Tg(Adipoq-cre)1Evdr	Prdm16 FKO	0	7	0

Animals/Strains

Experimental Factors

	Categorical Values
	Name / Abbreviation	Description
	Experimental Factor: Experimental Group
	Control	This animal belongs to the control group for the experiment.
	Experiment	This animal belongs to experimental group that is homozygous for gene manipulations.

Experimental Factor (Units)
Experimental Group

Experimental Factor Values

Phenotype Assays Add / Edit

Name	Abbreviation
glucose uptake	glucose uptake
average clamp glucose	average clamp glucose
fat body mass	fat body mass
lean body mass	lean body mass
total body weight	total body weight
glucose, basal	glucose, basal
hepatic glucose production, basal	hepatic glucose production, basal
glucose synthesis	glucose synthesis
glucose turnover, whole body	glucose turnover, whole body
glycogen/lipid synthesis, whole body	glycogen/lipid synthesis, whole body
glycolysis, whole body	glycolysis, whole body
glycogen synthesis	glycogen synthesis
glycolysis	glycolysis

No documents found.

Mammalian Phenotype (MP) Terms

MPTermID	MPTermName	Definition	Other Results
MP:0003926	decreased cellular glucose uptake	reduced ability of a cell to take in glucose from the environment [RGD:cur]	MMPC Search Results
MP:0005559	increased circulating glucose level	greater than the normal concentration in the blood of this major monosaccharide of the body; it is an important energy source [MGI:cwg\| RGD:cur]	MMPC Search Results
MP:0010024	increased total body fat amount	greater than the normal total amount of connective tissue composed of fat cells within the entire body [MGI:csmith]	MMPC Search Results
MP:0014142	increased body fat mass	increased physical bulk or volume of fat in the whole body [MGI:csmith]	MMPC Search Results

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