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Strain
C57BL/6-Eomestm1.1(cre/ERT2)Sjar Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J

Summary Data Summary
Official Name C57BL/6-Eomestm1.1(cre/ERT2)Sjar Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J
Common Name Tbr2 cond/cond
Description A cre/ERT2-polyA cassette and loxP-flanked neomycin
selection cassette were inserted into the Eomes locus at the
translational start site in exon 1 by homologous
recombination. This resulted in deletion of about 500 bp of
the coding region of exon 1 (creating a null allele). The
neo selection cassette was deleted in the final knockin
allele. In situ hybridization demonstrated that embryonic
expression of cre/ERT2 recapitulated the spatiotemporal
expression pattern of endogenous Eomes.

The Rosa-CAG-LSL-tdTomato-WPRE targeting vector was designed
with (from 5' to 3') a CMV-IE enhancer/chicken
beta-actin/rabbit beta-globin hybrid promoter (CAG), an FRT
site, a loxP-flanked STOP cassette (with stop codons in all
3 reading frames and a triple polyA signal), tdTomato
sequence (a non-oligomerizing DsRed fluorescent protein
variant with a 12 residue linker fusing two copies of the
protein (tandem dimer)), a woodchuck hepatitis virus
post-transcriptional regulatory element (WPRE; to enhance
the mRNA transcript stability), a polyA signal, and an
attB/attP-flanked PGK-FRT-Neo-polyA cassette. This entire
construct was inserted between exons 1 and 2 of the
Gt(ROSA)26Sor locus.
Development Status Phenotyping ongoing
Creation Method transgenic
TypeCount
Investigators 1
Genomics - Modifications 2
Experiments 2


Investigators
NameInstitution
David FeldheimUniversity of California-Santa Cruz


Genomic Information
GeneAllele 1Allele 2Protocol
Eomesknockin (Cre)knockin (Cre)Not Specified
Gt(ROSA)26SorknockinknockinNot Specified


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