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Strain
C57BL/6-Adora1tm1GreTg(CMV-HBV-Cre)

Summary Data Summary
Official Name C57BL/6-Adora1tm1GreTg(CMV-HBV-Cre)
Common Name C57BL/6-Adora1tm1GreTg(CMV-HBV-Cre)
Description The targeting vector for generating the inducible A1R
knock-out allele was constructed using plasmid
pB-Not-XhoMaxi (pMaxi), which contains the major coding exon
of the A1R [homologous to human exon 6; a gift from B.
Johansson. A loxP site along with HindIII and BamHI sites
were inserted into the EcoR1 site 5 to the exon. The 4.5 kb
fragment from plasmid pGB128 containing the cytosine
deaminase and neomycin resistance genes was inserted into
the NcoI site 3 to exon 6. This fragment was flanked by loxP
sites to allow removal of the cassette. All three loxP sites
in the final targeting vector were in the same 5 to 3
orientation. A 1.6 kb fragment from plasmid
pGKneobpAlox2PGKDTA containing the diphtheria toxin gene was
inserted into the XhoI site of pMaxi. The final targeting
vector was linearized with NotI and transfected into J1
embryonic stem (ES) cells derived from 129SvJ mice.
Development Status Phenotyping ongoing
Creation Method transgenic
TypeCount
Investigators 1
Genomics - Transgenes 1
Experiments 1


Investigators
NameInstitution
shanu jainNational Institutes of Health (NIH)


Genomic Information
EnhancerPromoterGeneCopies
Not SpecifiedHbviCRENot Specified


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