mmpc-logo mmpc-logo
twitter-logo    bluesky-logo
| Create Account | login
Strain
C57BL/6-Lmnatm1.1SchTg(Adipoq-cre/ERT2)1Soff/J

Summary Data Summary
Official Name C57BL/6-Lmnatm1.1SchTg(Adipoq-cre/ERT2)1Soff/J
Common Name C57BL/6-Lmnatm1.1SchTg(Adipoq-cre/ERT2)1Soff/J
Description LMNA gene are floxed at exons 10 and 11.
These transgenic mice express a tamoxifen-inducible cre
recombinase, Cre-ERT2, under the control of the Adipoq gene
promoter. They may be useful in generating
adipocyte-specific targeted mutants in studies related to
adipose tissue function and storage, obesity, and other
metabolic diseases.

A targeting vector containing cDNA encoding icre fused to
the mutated form of the ligand-binding domain of the human
estrogen receptor followed by an Frt-flanked ampicillin
resistance marker was generated. Homologous recombination
resulted in the insertion of the vector into the start codon
of the Adipoq locus present in a BAC clone (RP23-112M7; this
BAC also contained the genes Kng1, Eif4a2, and Rfc4.)
Flp-mediated excision removed the ampicillin marker. Five
founders were obtained and crossed with lacZ reporter mice.
Progeny of one founder displayed homogeneous lacZ expression
in epididymal, mesenteric, and retroperitoneal white adipose
tissue after tamoxifen treatment.
Development Status Phenotyping ongoing
Creation Method knockout
TypeCount
Investigators 1
Genomics - Modifications 1
Genomics - Transgenes 1
Experiments 8


Investigators
NameInstitution
Rebecca SchillUniversity of Michigan-Ann Arbor


Genomic Information
GeneAllele 1Allele 2Protocol
LmnaknockoutknockoutNot Specified
EnhancerPromoterGeneCopies
Not SpecifiedAdipoqCRENot Specified


Menu

Home
Contact
About MMPC
Animal Husbandry
Tests Data
Search Data
Analysis
Clients
MMPC Centers

Newsletter

Interested in receiving MMPC News?
twitter-logo Mouse Phenotyping
@NationalMMPC



2017 National MMPC. All Rights Reserved.