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Strain
C57BL/6-Agrptm1(cre)Lowl/J

Summary Data Summary
Official Name C57BL/6-Agrptm1(cre)Lowl/J
Common Name C57BL/6-Agrptm1(cre)Lowl/J
Description An IRES-cre-frt-kan/neo-frt cassette was inserted in frame
in exon 5 after the stop codon via homologous recombination.
The kan/neo cassette was subsequently removed via in vivo
Flp mediated recombination. Crosses to reporter mice
detected Cre activity in AGRP+ neurons. The mutation
resulted in a duplication of chromosome 8 genomic sequence
at least 53 kb in size that inserted into intron 1 of the
nearby Ripor1 (Fam65a) locus. The duplicated sequence
includes the entire Hsd11b2 locus, the entire Atp6v0d1 locus
and at least 11.7 kb of the Agrp locus. The insertion
appears to be in the ~9 kb intron upstream of the
translation start site-containing exon 2,however, the effect
on Ripor1 expression was not specifically analyzed.
Development Status Phenotyping ongoing
Creation Method knockin
TypeCount
Investigators 1
Genomics - Modifications 1
Experiments 6


Investigators
NameInstitution
Susan AllenUniversity of Michigan-Ann Arbor


Genomic Information
GeneAllele 1Allele 2Protocol
AgrpknockinknockinNot Specified


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