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Strain
STOCK Mc4rtm3.1(cre)Lowl/J

Summary Data Summary
Official Name STOCK Mc4rtm3.1(cre)Lowl/J
Common Name Mc4r-2a-Cre
Description A targeting construct was designed to insert a viral 2A
oligopeptide (T2A; to mediate ribosomal skipping) fused to a
Cre recombinase gene, followed by a frt-flanked neomycin
resistance (neo) cassette, in place of the stop codon for
the melanocortin 4 receptor (Mc4r) gene. The construct was
electroporated into 129S6/SvEvTac-derived W4 embryonic stem
(ES) cells. Correctly targeted ES cells were injected into
blastocysts and resulting chimeric mice were bred with
B6.Cg-Tg(ACTFLPe)9205Dym/J transgenic mice (Stock No.
005703) to delete the neo cassette. Progeny were bred with
C57BL/6 mice to remove the Flp-expressing transgene.
Resulting Mc4r-2a-Cre knock-in mice were maintained on a
mixed B6;129 genetic background. Upon arrival at The Jackson
Laboratory, sperm was cryopreserved. To establish our live
colony, an aliquot of frozen sperm was used to fertilize
C57BL/6J oocytes.
Development Status Phenotyping ongoing
Creation Method knockin
TypeCount
Investigators 1
Genomics - Modifications 1
Experiments 6


Investigators
NameInstitution
David OlsonUniversity of Michigan-Ann Arbor


Genomic Information
GeneAllele 1Allele 2Protocol
Mc4rknockinknockinNot Specified


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