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Strain
B6.Cg-Gt(ROSA)26Sortm14(CAG-tdTomato)HzeAgrptm1(cre)LowlTg (GHR-Cre)/J

Summary Data Summary
Official Name B6.Cg-Gt(ROSA)26Sortm14(CAG-tdTomato)HzeAgrptm1(cre)LowlTg (GHR-Cre)/J
Common Name AgRPdeltaGHR
Description An IRES-cre-frt-kan/neo-frt cassette was inserted in frame
in exon 5 after the stop codon via homologous recombination.
The kan/neo cassette was subsequently removed via in vivo
Flp mediated recombination. Crosses to reporter mice
detected Cre activity in AGRP+ neurons. The mutation
resulted in a duplication of chromosome 8 genomic sequence
at least 53 kb in size that inserted into intron 1 of the
nearby Ripor1 (Fam65a) locus. The duplicated sequence
includes the entire Hsd11b2 locus, the entire Atp6v0d1 locus
and at least 11.7 kb of the Agrp locus. The insertion
appears to be in the ~9 kb intron upstream of the
translation start site-containing exon 2,however, the effect
on Ripor1 expression was not specifically analyzed.
Adult male and female AgRPtm1(cre) (AgRP-Ires-cre, stock
012899) were purchased from
The Jackson Laboratory, and GHRL/L mice were described
previously [19]. The characterization
of the AgRP?GHR mice was described previously [18
Development Status Phenotyping ongoing
Creation Method knockin
TypeCount
Genomics - Modifications 1
Genomics - Transgenes 1
Experiments 4


Genomic Information
GeneAllele 1Allele 2Protocol
Agrpknockin (Cre)knockin (Cre)Not Specified
EnhancerPromoterGeneCopies
Not SpecifiedGHRCRENot Specified


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