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Experiment

2020-07-20 Marianna Sadagurski 12 Mice CLAMS Part 3 22-30C

Summary

Data Summary

Investigator	Sadagurski, Marianna
Description	The current work aims to delineate key interactions among hormones (leptin, insulin, GH) and the brain that are involved in the regulation of feeding behavior, glucose metabolism, and body composition. It is clear that communication between the periphery and the brain is key for normal body weight and glucose levels. Consequently, dysfunction in this system is directly related to the cause of obesity and type 2 diabetes (a disease where blood sugar levels cannot be controlled normally). It is our goal to gain an understanding of the physiology, and pathophysiology of energy and glucose homeostasis, and ultimately to devise better treatments for obesity and diabetes. Since leptin is a crucial signal to the neural mediators of energy balance, we employ leptin to dissect these neural mediators, both by examining roles for specific leptin signals (signaling proteins, neuropeptides, etc.) and by identifying and understanding the function of leptin responsive neurons. Leptin can modulate growth hormone (GH) secretion and the GH central responses. In our recent study, we found that leptin receptor-expressing neurons (LepRb) represent the crucial locus for GH signaling to modulate metabolism and specifically hepatic glucose production. Understanding the mechanisms of this modulation will aid in understanding the mechanisms of hypoglycemia that may be important for therapeutic intervention. We will understand the roles for specific leptin and GH signals and other signaling proteins that operate downstream of LepRb and GHR) in energy balance and metabolism. We will evaluate the contributions of these proteins to neural function, feeding, and other physiologic parameters (including neuroendocrine function) in neuronal populations that express both LepRb and GHR. Mice are an excellent model for human food intake, energy expenditure, and body weight regulation. They are omnivores like humans and their brain control systems that are similar to those found in humans. Conseque
Status	Completed
Public Release	9/15/2024
Animal Age	Measured In: week(s) post-natal (w)
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Data Analysis

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Type	Count
Animals	12
Experimental Conditions	8
Catalog Items	1
Curation Info (# flags)	1
Phenotype Assays	2
Phenotype Measurements	8136
Histology Images	0
Publications	0

Animals

Strain Name	Common Name	Females	Males	Unknown
B6.Cg-Gt(ROSA)26Sor^{tm14(CAG-tdTomato)Hze}Agrp^tm1(cre)LowlTg (GHR-Cre)/J	AgRPdeltaGHR	0	12	0

Animals/Strains

Experimental Factors

	Categorical Values
	Name / Abbreviation	Description
	Experimental Factor: Drug Administered
	Saline	Saline
	Experimental Factor: Drug Delivery Route
	IP	The drug was delivered intraperitoneal.
	Experimental Factor: Experimental Group
	Control	This animal belongs to the control group for the experiment.
	Experiment	This animal belongs to experimental group that is homozygous for gene manipulations.
	Experimental Factor: Mouse Diet
	LabDiet PicoLab Rodent Diet 5LOD	Crude Protein 23%, crude fat 4.5%, crude fiber 6.0%. Ingredients: dehulled soybean meal, ground corn, dried beet pulp, fish meal, ground oats, dehydrated alfalfa meal, cane molasses, brewers dried yeast, wheat germ, whey, porcine animal fat...
	Experimental Factor: Second Drug Administered
	Clozapine N-oxide	The second drug that was administered during the study.
	Experimental Factor: Strain Type
	Heterozygous	Having two different alleles of a particular gene or genes
	Homozygous	Having two identical alleles of a particular gene or genes.

Experimental Factor (Units)
Drug Administered
Drug Delivery Route
Environmental Temperature (°C) (°C)
Experimental Group
Mouse Diet
Second Drug Administered
Strain Type
Time Sample Taken (min)

Experimental Factor Values

Phenotype Assays Add / Edit

Name	Abbreviation
respiratory exchange ratio	RQ
VO2 rate	VO2 rate

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