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Case Western Reserve University (Alumni MMPC)
http://www.case.edu/med/mmpc/index.html

Description Provides isotopic measurement of metabolic pathway fluxes such as lipid and
protein turnover using mass spec.
Director / Email Henri Brunengraber, M.D., Ph.D. - henri.brunengraber@case.edu
General Contact mmpc@case.edu
Grant Number DK076174
RRID (Cite This)

Center Cores:

 Center CoreCore DescriptionRRIDInactive?

Center: Case Western Reserve University; Director: Henri Brunengraber, M.D., Ph.D.

 Analytical Core (cwru)The analytical Core conducts all assays involving the determination of isotope labeling and concentration profiles of metabolites including (i) the 2H-labeling of water, lipids and amino acids, (ii) the 18O-labeling of water, (iii) the concentration profile of acyl-CoAs (e.g. short, medium and long-chain species), and (iv) metabolic profiles of citric acid cycle intermediates, amino acids and fatty acids. Active
 Metabolic CoreThe Metabolic Core conducts in vivo and ex vivo metabolic experiments on mice shipped to the Case MMPC. This core focuses on the following techniques and measurements: 1) Chronic or acute arterial, jugular and/or gastric catheterization 2) Acute catheterization of portal vein or urinary bladder 3) Energy expenditure integrated over four days by the "doubly-labeled water" method 4) Rates of fatty acid, cholesterol, triglycerides, or protein synthesis measured using 2H2O 5) Insulin clamp, pancreatic clamp 6) Food intake 7) Body temperature 8) Urine and blood chemistry analysis 9) Glycosylated hemoglobin 10) Liver perfusion and heart perfusion 11) Tissue perfusion and fixation Active

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