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University of Texas Southwestern Medical Center (Alumni MMPC)
http://www.utsouthwestern.edu/utsw/cda/dept281587/files/576302.html

Description Provides isotopic measurement of metabolic pathway fluxes such as TCA cycle and
gluconeogenesis using NMR.
Director / Email Craig Malloy, M.D. - craig.malloy@utsouthwestern.edu
General Contact shawn.burgess@utsouthwestern.edu
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 Center CoreCore DescriptionRRIDInactive?

Center: University of Texas Southwestern Medical Center; Director: Craig Malloy, M.D.

 Rogers NMR Center Metabolic CoreThe Rogers NMR Center Mouse Metabolic Phenotyping Center specializes in the use of stable isotopes for investigations of metabolism, including a comprehensive analysis of gluconeogenesis. Our goal is to provide a repertoire of metabolic analyses that can be standardized and used to phenotype mouse models of type 2 diabetes. These tools are based on new concepts for probing intermediary metabolism using 13C- or 2H-enriched compounds. In some instances the studies are simple enough to perform in the referring investigator's lab; only partially processed tissue samples need be shipped to the Rogers NMR Center for full analysis. Procedures are available for determining the substrates contributing to plasma glucose, measurement of both the relative and absolute rates of the pathways involved in gluconeogenesis, substrate utilization in the heart, and 23Na/31P NMR investigations of liver metabolism. In addition, software is made available to predict the potential effects of genetic manipulation on various pathways. Active

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