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University of Washington, Seattle (Alumni MMPC)
http://www.mmpc.washington.edu/

Description Tests for diabetic complications (micro and macrovasculature, heart, eye,
kidney) and measures energy balance, eating behavior and activity.
Director / Email Renee LeBoeuf, Ph.D. - leboeuf@u.washington.edu
General Contact geronimo@u.washington.edu
Grant Number
RRID (Cite This)

Center Cores:

 Center CoreCore DescriptionRRIDInactive?

Center: University of Washington, Seattle; Director: Renee LeBoeuf, Ph.D.

 Cardiovascular CoreDescription of Services: 1) Cardiovascular injury models (myocardial infarction, hindlimb ischemia and collateralization. 2) Structural and physiological assessment of organ damage that reflect common complications of diabetes. 3) Flow cytometric quantitation of circulating progenitor cells (e.g. c-kit, sca-1, Flt-1, and CD31). 4) Bioimmunoassay analysis of growth factors, cytokines, and chemokines affecting the cardiovascular system in diabetes and in injury. Active
 Diabetes and Energy Balance CoreDescription of Services * Precise, non-invasive, quantitative assessment of energy homeostasis in rodent models including energy expenditure, respiratory quotient, locomotor activity, body temperature, food intake, meal patterns and body composition. * Assessment of a variety of metabolic disease phenotypes associated with obesity and type 2 diabetes such as glucose intolerance, insulin resistance and hyperlipidemia in mice. * Quantification of the extent of insulitis and other inflammatory parameters in mouse models of type 1 diabetes mellitus such as autoimmune NOD and hyperglycemic Akita mice and mice treated with pancreatic islet toxins. * Quantification using PCR approaches of the expression of genes in the hypothalamus and other sites involved in food intake, energy expenditure, and tissue complications of diabetes. * Quantification of circulating and/or tissue factors (e.g. hormones, proteins, lipids, and carbohydrates) associated with the metabolic syndrome, obesity, type 1 diabetes, or type 2 diabetes. * Longitudinal studies: To perform longitudinal studies in single animals as a function of age, diet or special treatment regimes using specific drugs or islet toxins. Active
 Nephrology, Macrovascular and Microvascular CoreDescription of Services 1) Blood pressure, plasma lipid and lipoprotein levels, urine albumin. 2) Processing (freezing and/or fixation and paraffin embedding) of tissue or cells. 3) Sectioning of frozen and paraffin-embedded tissue blocks appropriate for light, fluorescent and electron microscopy. 4) Diagnostic pathology expertise in the evaluation of local and systemic effects in tissues and target organs of diabetic animals. 5) Monoclonal and pojyclonal antibodies to targeted gene products and reporter genes (e.g., p-galactosidase, alkaline phosphatase, green fluorescent protein). 6) RNA and DNA probes for in situ hybridization studies, which complement studies of expressed proteins. Active

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