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Experiment

Metabolic measurements in B6 mice fed high fat diet and treated with fruit
extracts

Summary

Data Summary

Investigator	Shengkan, Jin
Description	5 week old male C57BL/6J mice were treated with high fat diet(HFD) or HFD plus 0.25% fruit extracts, or HFD plus 0.5% fruit extracts for 15 weeks. After 5 hour fasting, mice were anesthetized with ketamine and xylazine to draw blood from heart with heparin-coated tube. Plasma was obtained by centrifugation at 1000g for 10-15min at 4? , and stored at -80? for further analysis. Applicable research area(s): Diabetes, Obesity, Metabolism
Status	Completed
Public Release	8/20/2017
Animal Age	Measured In: week(s) post-natal (w)
Flags

Data Analysis

DOWNLOAD ALL DATA (.csv)

Type	Count
Animals	18
Experimental Conditions	5
Catalog Items	5
Curation Info (# flags)	1
Phenotype Assays	5
Phenotype Measurements	90
Histology Images	0
Publications	0

Animals

Strain Name	Common Name	Females	Males	Unknown
C57BL/6	C57BL/6	0	18	0

Animals/Strains

Experimental Factors

	Categorical Values
	Name / Abbreviation	Description
	Experimental Factor: Experimental Group
	Control	This animal belongs to the control group for the experiment.
	Experiment	This animal belongs to experimental group that is homozygous for gene manipulations.
	Experimental Factor: Feeding Group
	Fasted	Animals are deprived of food but not water.
	Experimental Factor: Mouse Diet
	Research Diets - D12492	Rodent Diet with 34% Fat, 60% kcal from Fat
	Research Diets - D12492 0.25% fruit	Rodent Diet with 60% fat, 20% carbohydrate, 20% protein supplemented with 0.25% fruit extract
	Research Diets - D12492 0.5% fruit	Rodent Diet with 60% fat, 20% carbohydrate, 20% protein supplemented with 0.5% fruit extract
	Experimental Factor: Tissue Source
	plasma (lithium heparin)	plasma (lithium heparin)

Experimental Factor (Units)
Experimental Group
Fast Duration (hr)
Feeding Group
Mouse Diet
Tissue Source

Experimental Factor Values

Phenotype Assays Add / Edit

Name	Abbreviation
cholesterol, total	TC
triglycerides	TG
non-esterified free fatty acids	NEFA
alanine aminotransferase	ALT
aspartate aminotransferase	AST

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