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Hyperinsulinemic Euglycemic clamp order #18590, November 2015
Summary Data Summary
Investigator Wheatcroft, Stephen
Description We have found in preliminary experiments that infusion of a small RGD peptide to
wild type C57BL/6 mice on an obesogenic diet leads to improved glucose tolerance
and increased insulin sensitivity on GTT and ITTs.

Purchase 8 week old male C57BL6 mice
Commence high fat diet (F3282 BioServ, NJ) ad libitum at 8 weeks old
After 4 weeks on diet implant s/c minipumps infusing RGD peptide or scrambled
control peptide (to be supplied by investigator) over 4 weeks
Continue high fat diet
During week 4 of infusion (week 8 on diet) undertake glucose clamping

Applicable research area(s): Diabetes, Metabolism
Status Completed
Public Release 1/7/2018
Animal Age Measured In: week(s) post-natal (w)
Flags has-data-flagSame StrainAnimal CountNo data
Data Analysis
Experimental Conditions6
Catalog Items3
Curation Info (# flags)3
Phenotype Assays16
Phenotype Measurements5145
Histology Images0

Strain NameCommon NameFemalesMalesUnknown

Experimental Factors
 Categorical Values
Name / AbbreviationDescription

Experimental Factor: Drug Administered

 Peptide - Control
Control peptide
 Peptide - RGD
RGD Peptide

Experimental Factor: Experimental Group

This animal belongs to the control group for the experiment.
This animal belongs to experimental group that is homozygous for gene manipulations.

Experimental Factor: Glucose Infusion Rate (mg/kg/min)

 Glucose Infusion Rat
Rate of glucose infusion for clamp experiments

Experimental Factor: Insulin Infusion Rate - milliUnits (mU/kg/min)

 Insulin Infusion Rat
This is insulin infusion rate for hyperinsulinemic clamp data

Experimental Factor: Mouse Diet

 BioServ F3282
Rodent Hig Fat Diet. Protein 20.%, Fat 35.5%, Fiber 0.0%, Ash 3.2%, Moisture <5.0%, Carbohydrate 36.3%. Ingredients: Lard, Casein, Dextrose, Sucrose, DL-Methionine, Choline Chloride, Vitamin Mix and Mineral Mix

Experimental Factor: Time Sample Taken (min)

 Time Sample Taken
Time in minutes since a sample was taken

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