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Metabolic measurements in HFD fed B6 mice treated with niclosamide and metformin
Summary Data Summary
Investigator Shengkan, Jin
Description We had the C57BL6/J mice randomized into 4 groups. These mice were all fed on
high-fat diet (the Research Diets Inc. D12492) for 12 weeks. Group 1 is controls
without any treatment, including mice C1-C8. Group 2 is niclosamide treatment
group with oral treatment of niclosamide mixed into chow 2000ppm, including mice
N1-N8. Group 3 in metformin treatment group with oral treatment of metformin in
water to achieve dosage of 75 mg/kg/d, including mice M1-M8. Group 4 is
synergistic treatment group with both niclosamide and metformin treatment. Blood
samples were collected at age of 24 weeks after 16 hour fast. Each sample
contains ~50 micro liters of plasma.

Mice homozygous for the Apoetm1Unc mutation show a marked increase in total
plasma cholesterol levels that are unaffected by age or sex. Fatty streaks in
the proximal aorta are found at 3 months of age. The lesions increase with age
and progress to lesions with less lipid but more elongated cells, typical of a
more advanced stage of pre-atherosclerotic lesion. Moderately increased
triglyceride levels have been reported in mice with this mutation on a mixed
C57BL/6 x 129 genetic background. Aged apoE-deficient mice (>17 months) have
been shown to develop xanthomatous lesions in the brain consisting mostly of
crystalline cholesterol clefts, lipid globules, and foam cells. Smaller
xanthomas were seen in the choroid plexus and ventral fornix. Additional studies
indicate that apoE-deficient mice have altered responses to stress, impaired
spatial learning and memory, altered long term potentiation, and synaptic

Applicable research area(s): Cardiovascular, Metabolism
Status Completed
Public Release 5/20/2018
Animal Age Measured In: week(s) post-natal (w)
Flags has-data-flagSame Strain
Data Analysis
Experimental Conditions8
Catalog Items4
Curation Info (# flags)1
Phenotype Assays5
Phenotype Measurements160
Histology Images0

Strain NameCommon NameFemalesMalesUnknown

Experimental Factors
 Categorical Values
Name / AbbreviationDescription

Experimental Factor: Drug Administered

Metformin is a drug approved by the U.S. Food and Drug Administration as a prescription medication to treat diabetes. This medication is used to decrease hepatic (liver) glucose production, to decrease GI glucose absorption and to increase target...
Vehicle - Control

Experimental Factor: Drug Dose Units

milligrams per kilograms per day

Experimental Factor: Experimental Group

This animal belongs to the control group for the experiment.
This animal belongs to experimental group that is homozygous for gene manipulations.

Experimental Factor: Feeding Group

Animals are deprived of food but not water.

Experimental Factor: Mouse Diet

 Research Diets - D12492
Rodent Diet with 34% Fat, 60% kcal from Fat
 Research Diets - D12492 2000 ppm NEN
Rodent Diet with 60% fat, 20% carbohydrate, 20% protein supplemented with 2000 ppm niclosamide ethanolamine (NEN)

Experimental Factor: Tissue Source

 plasma (EDTA)
plasma (EDTA)
Experimental Factor Values

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