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Experiment

Sable Energy Balance #22069 10/14/16
Summary Data Summary
Investigator Simerly, Richard
Description Energy expenditure measured by indirect calorimetry in the Promethion by Sable
Systems. Activity, food intake and water intake are also continuously measured.
Measurements were taken for 8 consecutive days.
Running wheels were not present in the cage.
Mice were fed ad libitum.
Water: regular tap water, ad libitum.
Mice had head cannula which were used to give 1 nmol AgRP peptide to each mouse.

Groups:
Male WT (C57BL/6J ) and Tg (C57BL/6-Kcnj13tm1c(KOMP)Wtsi Tg(Mc4r-Cre)) mice.
Mice were fed a chow diet (LabDiet PicoLab Rodent Diet 5LOD).
Male WT Chow: n=2,
Male Tg Chow: n=2,

Purpose of the experiment: This experiment will determine whether the groups of
mice have different energy expenditure and feeding behaviors.

Applicable Research Areas: Metabolism , Body weight regulation, energy
expenditure, feeding behavior.
Status Completed
Public Release 11/10/2018
Animal Age Measured In: day(s) post-natal (d)
Flags has-data-flagExperiment Description
Data Analysis
TypeCount
Animals4
Experimental Conditions3
Catalog Items1
Curation Info (# flags)1
Phenotype Assays13
Phenotype Measurements52
Histology Images0
Publications0


Animals
Strain NameCommon NameFemalesMalesUnknown
C57BL/6J
0
2
0
C57BL/6-Kcnj13tm1a(KOMP)Wtsi Tg(Mc4r-Cre)
0
2
0

Animals/Strains
Experimental Factors
 Categorical Values
Name / AbbreviationDescription

Experimental Factor: Drug Administered

 AgRP Peptide
Agouti-related peptide(AgRP) is a neuropeptide produced in the brain by the AgRP/NPY neuron. AgRP is a paracrine signalling molecule made up of 112 amino acids (the gene product of 132 amino acids is processed by removal of the N-terminal...

Experimental Factor: Experimental Group

 Control
This animal belongs to the control group for the experiment.
 Experiment
This animal belongs to experimental group that is homozygous for gene manipulations.

Experimental Factor: Mouse Diet

 LabDiet PicoLab Rodent Diet 5LOD
Crude Protein 23%, crude fat 4.5%, crude fiber 6.0%. Ingredients: dehulled soybean meal, ground corn, dried beet pulp, fish meal, ground oats, dehydrated alfalfa meal, cane molasses, brewers dried yeast, wheat germ, whey, porcine animal fat...
Experimental Factor (Units)
Drug Administered
Experimental Group
Mouse Diet
Experimental Factor Values


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