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Tracers in Metabolic Research
Isotope Tracers in Metabolic Research: 3-Part Webinar Series
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The L1L2_Bact_P cassette was inserted at position 87389830
of Chromosome 1 upstream of the critical exon(s) (Build
GRCm38). The cassette is composed of an FRT site followed by
lacZ sequence and a loxP site. This first loxP site is
followed by neomycin under the control of the human
beta-actin promoter, SV40 polyA, a second FRT site and a
second loxP site. A third loxP site is inserted downstream
of the targeted exon(s) at position 87388688. The critical
exon(s) is/are thus flanked by loxP sites. A "conditional
ready" (floxed) allele was then created by crossing with a
flp recombinase expressing mouse carrying this allele.
Subsequent cre expression results in a knockout mouse. For
this strain, the floxed Kcnj13 tm1a strain was crossed with
a mouse strain where the Mc4r promoter drives the Cre gene.
Genomics - Modifications
Genomics - Transgenes
Curation Flag Information
New comment to be added:
Sable Energy Balance # 22069 12/5/16
Sable Energy Balance #22039 9/8/16
Sable Energy Balance #22069 10/14/16
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Financial support for this work was provided by the NIDDK Mouse Metabolic Phenotyping Centers (National MMPC, RRID:SCR_008997,
) under the MICROMouse Program, grants DK076169.
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