mmpc-logo
twitter-logo @NationalMMPC
| Create Account | login
Strain
C57BL/6-Kcnj13tm1a(KOMP)Wtsi Tg(Mc4r-Cre)25Rck

Summary Data Summary
Official Name C57BL/6-Kcnj13tm1a(KOMP)Wtsi Tg(Mc4r-Cre)25Rck
Common Name C57BL/6-Kcnj13tm1a(KOMP)Wtsi Tg(Mc4r-Cre)
Description The L1L2_Bact_P cassette was inserted at position 87389830
of Chromosome 1 upstream of the critical exon(s) (Build
GRCm38). The cassette is composed of an FRT site followed by
lacZ sequence and a loxP site. This first loxP site is
followed by neomycin under the control of the human
beta-actin promoter, SV40 polyA, a second FRT site and a
second loxP site. A third loxP site is inserted downstream
of the targeted exon(s) at position 87388688. The critical
exon(s) is/are thus flanked by loxP sites. A "conditional
ready" (floxed) allele was then created by crossing with a
flp recombinase expressing mouse carrying this allele.
Subsequent cre expression results in a knockout mouse. For
this strain, the floxed Kcnj13 tm1a strain was crossed with
a mouse strain where the Mc4r promoter drives the Cre gene.
Development Status Phenotyping ongoing
Creation Method knockout
Background C57BL/6
Breeding Type intercross
TypeCount
Investigators 1
Genomics - Modifications 1
Genomics - Transgenes 1
Experiments 3


Investigators
NameInstitution
Richard SimerlyVanderbilt University


Genomic Information
GeneAllele 1Allele 2Protocol
Kcnj13knockin (lox)knockin (lox)Not Specified
EnhancerPromoterGeneCopies
Not SpecifiedMc4rCRENot Specified


Menu

Home
Contact
About MMPC
Animal Husbandry
Tests Data
Search Data
Analysis
Clients
MMPC Centers

Newsletter

Interested in receiving MMPC News?
twitter-logo Mouse Phenotyping
@NationalMMPC



2017 National MMPC. All Rights Reserved.