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Impact of High Fat Diet Choice on Energy Balance

Summary

Data Summary

Investigator	Johnson, Carl
Description	The impact of high fat diet choice on energy balance. Animals eating at night gain more weight than animals eating during the day. Circadian rhythm study, lights were on from 10 AM-10 PM and lights were off from 10 PM-10 AM. There were four groups of mice with different diet options. Mice from Group 1 were given only chow diet. They had access to this diet at all times. Mice from Group 2 were given both chow diet and high fat diet. They had access to these diets at all times. Group 3 was given both chow diet and high fat diet. They had access to the chow diet at all times. They could only access the high fat diet from 10 PM-4 AM. Group 4 was given both chow diet and high fat diet. They had access to the chow diet at all times. They could only access the high fat diet from 4 AM-10 AM. During run 1 the mice only had access to chow. An extra hopper of the chow diet was provided at this time as a substitute for the high fat diet. The extra hopper was blocked at the times listed above for the high fat diet for Group 3 and Group 4. High fat was added to the appropriate groups during run 2 and was continued through run 3. Group 1 kept the extra chow diet hopper during run 2 and run 3. The weights for the entire experiment are an average of the weight at the start of run 1 and the weight at the end of run 3. The same applies to the lean mass, fat mass, and free body water mass. Applicable research area(s): Metabolism
Status	Completed
Public Release	1/13/2019
Animal Age	Measured In: day(s) post-natal (d)
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Data Analysis

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Type	Count
Animals	16
Experimental Conditions	7
Catalog Items	1
Curation Info (# flags)	1
Phenotype Assays	13
Phenotype Measurements	1248
Histology Images	0
Publications	0
Phenotypes	1

Animals

Strain Name	Common Name	Females	Males	Unknown
C57BL/6J	C57BL/6J	0	16	0

Animals/Strains

Experimental Factors

	Categorical Values
	Name / Abbreviation	Description
	Experimental Factor: Experimental Group
	Control	This animal belongs to the control group for the experiment.
	Experiment	This animal belongs to experimental group that is homozygous for gene manipulations.
	Experimental Factor: Mouse Diet
	LabDiet PicoLab Rodent Diet 5LOD	Crude Protein 23%, crude fat 4.5%, crude fiber 6.0%. Ingredients: dehulled soybean meal, ground corn, dried beet pulp, fish meal, ground oats, dehydrated alfalfa meal, cane molasses, brewers dried yeast, wheat germ, whey, porcine animal fat...
	Research Diets - D12492	Rodent Diet with 34% Fat, 60% kcal from Fat

Experimental Factor (Units)
Experimental Group
Food Ingestion (g/day)
Mouse Diet
Mouse Diet Hopper (unitless)
Mouse Feeding - Start (unitless)
Mouse Feeding - Stop (unitless)
Sable Run (RNum) (RNum)

Experimental Factor Values

Phenotype Assays Add / Edit

Name	Abbreviation
body mass	W rodent
oxygen consumption (Light Period)	VO2-L
fat body mass	fat body mass
lean body mass	lean body mass
respiratory exchange ratio (Light Period)	RER-L
body water, free	free water
heat (Light Period)	HEAT-Light
oxygen consumption (Dark Period)	VO2-D
carbon dioxide production Light Period	VCO2-L
carbon dioxide production Dark Period	VCO2-D
respiratory exchange ratio (Dark Period)	RER-D
heat (Dark Period)	HEAT-Dark
temperature, environmental	ENV TEMP

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Mammalian Phenotype (MP) Terms

MPTermID	MPTermName	Definition	Other Results
MP:0010379	decreased respiratory quotient	reduction in the ratio of the volume of carbon dioxide released to oxygen consumed by a body tissue or an organism compared to controls [MGI:csmith]	MMPC Search Results

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