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Experiment

Impact of High Fat Diet Choice on Energy Balance
Summary Data Summary
Investigator Johnson, Carl
Description The impact of high fat diet choice on energy balance. Animals eating at night
gain more weight than animals eating during the day.

Circadian rhythm study, lights were on from 10 AM-10 PM and lights were off from
10 PM-10 AM. There were four groups of mice with different diet options.

Mice from Group 1 were given only chow diet. They had access to this diet at all
times.

Mice from Group 2 were given both chow diet and high fat diet. They had access
to these diets at all times.

Group 3 was given both chow diet and high fat diet. They had access to the chow
diet at all times. They could only access the high fat diet from 10 PM-4 AM.

Group 4 was given both chow diet and high fat diet. They had access to the chow
diet at all times. They could only access the high fat diet from 4 AM-10 AM.

During run 1 the mice only had access to chow. An extra hopper of the chow diet
was provided at this time as a substitute for the high fat diet. The extra
hopper was blocked at the times listed above for the high fat diet for Group 3
and Group 4. High fat was added to the appropriate groups during run 2 and was
continued through run 3. Group 1 kept the extra chow diet hopper during run 2
and run 3.

The weights for the entire experiment are an average of the weight at the start
of run 1 and the weight at the end of run 3. The same applies to the lean mass,
fat mass, and free body water mass.

Applicable research area(s): Metabolism
Status Completed
Public Release 1/13/2019
Animal Age Measured In: day(s) post-natal (d)
Flags has-data-flagSame Strain
Data Analysis
TypeCount
Animals16
Experimental Conditions7
Catalog Items1
Curation Info (# flags)1
Phenotype Assays13
Phenotype Measurements1248
Histology Images0
Publications0
Phenotypes1


Animals
Strain NameCommon NameFemalesMalesUnknown
C57BL/6J
0
16
0

Experimental Factors
 Categorical Values
Name / AbbreviationDescription

Experimental Factor: Experimental Group

 Control
This animal belongs to the control group for the experiment.
 Experiment
This animal belongs to experimental group that is homozygous for gene manipulations.

Experimental Factor: Food Ingestion (g/day)

 food ingestion
The amount of chow, in grams, consumed by a mouse in one day.

Experimental Factor: Mouse Diet

 LabDiet PicoLab Rodent Diet 5LOD
Crude Protein 23%, crude fat 4.5%, crude fiber 6.0%. Ingredients: dehulled soybean meal, ground corn, dried beet pulp, fish meal, ground oats, dehydrated alfalfa meal, cane molasses, brewers dried yeast, wheat germ, whey, porcine animal fat...
 Research Diets - D12492
Rodent Diet with 34% Fat, 60% kcal from Fat

Experimental Factor: Mouse Diet Hopper (unitless)

 mouse diet hopper
For mouse experiments that provide mouse diet options for the animal. This number for the chow hopper that contained a specific mouse diet.

Experimental Factor: Mouse Feeding - Start (unitless)

 mouse feeding - star
This is the start time of day a mouse feeding hopper is available to the animal. The time is in military time, e.g. 2200 = 10PM.

Experimental Factor: Mouse Feeding - Stop (unitless)

 mouse feeding - stop
This is the stop time of day a mouse feeding hopper is available to the animal. The time is in military time, e.g. 2200 = 10PM.

Experimental Factor: Sable Run (RNum)

 Sable Run
The run number for the Sable system used to assess energy expenditure.


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