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Experiment

Influence of control or western diet on brain metabolites in C57bl/6 or LDLr-/-
mice
Summary Data Summary
Investigator Rutledge, John
Description Recent work suggests that diet and its effect on systemic and brain metabolism
impacts cognitive function and predisposition to dementia. We hypothesized that
diet or genetic predisposition to hyperlipidemia perturbs brain metabolism and
induces neuroinflammation that impairs cognitive performance. Therefore we first
aim to determine if and how western diet and/or LDLr-/- influence brain
metabolites. C57BL/6 or LDLr-/- mice are subjected to either a Western diet or
control diet starting at 8 weeks of age for 3 months. Frozen brain hemisphere
samples from 4 mice per group (16 total) will be subjected to LC-MS, GC-MS to
determine metabolic profiles including: biogenic amines, primary metabolites,
complex lipids, and oxylipins.
Status Completed
Public Release 6/16/2019
Animal Age Measured In: week(s) post-natal (w)
Flags has-data-flagSame Strain
Data Analysis
TypeCount
Animals16
Experimental Conditions2
Catalog Items2
Curation Info (# flags)1
Phenotype Assays1
Phenotype Measurements16
Histology Images0
Publications0
Documents4


Animals

Strain NameCommon NameFemalesMalesUnknown
C57BL/6J
0
11
0
C57BL/6J-Ldlr-/-
0
5
0

Experimental Factors
 Categorical Values
Name / AbbreviationDescription

Experimental Factor: Experimental Group

 Control
This animal belongs to the control group for the experiment.
 Experiment
This animal belongs to experimental group that is homozygous for gene manipulations.

Experimental Factor: Mouse Diet

 Teklad - 08485
Harlan Teklad TD.08485 Low Fat Control Diet Purified Diet, Modification of TD.88137, Low Fat & No Added Cholesterol, Reduced Sucrose.
 Teklad - 88137
Harlan Teklad TD.88137 (Western Diet)
Experimental Factor (Units)
Experimental Group
Mouse Diet


Phenotype Assays Add / Edit



No documents found.

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