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Strain

B6.129S7-Ldlr^tm1Her/J

Summary

Data Summary

Official Name	B6.129S7-Ldlr^tm1Her/J
Common Name	C57BL/6J-Ldlr^-/-
Description	Insertion of a neomycin resistance cassette into exon 4. The authors predict that the targeted allele would encode a truncated non-functional protein that will not bind LDL, and that lacks a membrane spanning segment. Immunoblot analysis of liver membranes detected a truncated protein in homozygous mutant animals. Mice homozygous for the Ldlrtm1Her mutation have an elevated serum cholesterol level of 200-400 mg/dl and they have very high levels (>2,000 mg/dl) when fed a high fat diet. Normal serum cholesterol in the mouse is 80-100 mg/dl.
Creation Method	knockout
Background	C57BL/6J
Breeding Type	intercross
Breeding Generations	10
Phenotype Description	Strain Development The Ldlrtm1Her mutant strain was developed in the laboratory of Dr. Robert Hammer and Dr. Joachim Herz at the Howard Hughes Medical Institute Research Laboratories, University of Texas Southwestern Medical Center at Dallas. The 129-derived AB1 ES cell line was used. C57BL/6J mice were generated by crossing the Ldlrtm1Her mutation 5 or more times to C57BL/6J mice.

Type	Count
Genomics - Modifications	1
Child Strains	3
Experiments	17
Publications	2

Genomic Information

Gene	Allele 1	Allele 2	Protocol
Ldlr	knockout	knockout	Not Specified

Strain	Development Status	Creation Method
C57BL/6-Tg(Csf1r-EGFP-NGFR/FKBP1A/TNFRSF6)2Bck Ldlr^tm1Her	Phenotyping ongoing	not applicable
B6.129S7-Adipoq^tm1Pesch Ldlr^tm1Her	Phenotyping ongoing	knockout
B6.129S7-Adipoq^tm1Pesch/+ Ldlr^tm1Her	Phenotyping ongoing	knockout

OrderID	Order	Investigator	Experiment	Status	Measurements
19665	19665	Linda Lee	BBB permeability in LDLr and COntrol mice on Western diet	Completed	32
620	620	Alyssa Hasty	Body composition - V3016 PXN1836	Completed	51
25879	25879	Amparo Villablanca	Circulating glucose, insulin, and estradiol levels in Ldlr KO mice fed high fat diet	Completed	36
25935	25935	Amparo Villablanca	Circulating glucose, insulin, and lipid levels in Ldlr KO mice fed high fat diet	Completed	72
18501	18501	Robin Altman	Circulating lipid levels in Ldlr KO mice fed high fat diet	Completed	72
13231	13231	Jennifer Rutkowsky	Circulating lipids in LDLr KO mice	Completed	128
21760	21760	John Rutledge	Cognitive assessment of c57BL/6 and LDLr -/- mice on a western diet	Completed	3240
30461	30461	Amparo Villablanca	Cognitive effects of western diet in female c57BL/6J and LDLR -/- mice.	Completed	20
30461	30461	Amparo Villablanca	Effect of western diet on open field performance in C57BL/6J and LDLR -/- mice	Completed	80
1810	1810	Laurie Minze	Experiment for Order #1810	Completed	186
1815	1815	Laurie Minze	Experiment for Order #1815	Completed	54
13321	13321	Jennifer Rutkowsky	Histological analysis of C57BL6 and LDLr KO mice fed on low fat vs high fat diet	Completed	132
1409	1409	Shobha Ghosh	Hyperinsulinemic-Euglycemic Clamp Order #1409 7/20/07	Completed	4531
22014	22014	John Rutledge	Influence of control or western diet on brain metabolites in C57bl/6 or LDLr-/- mice	Completed	16
18501	18501	Robin Altman	Liver lipids in Ldlr KO mice	Completed	36
13264	13264	John Rutledge	Plasma metabolomics in non-fasted control and Western diet fed WT and LDLRKO mice	Completed	40
22339	22339	Thao Luu	Sable Energy Balance #22339 8/3/17	Completed	224

Publication	Altmetrics	Submitted By	PubMed ID	Status
Rosiglitazone attenuates age- and diet-associated nonalcoholic steatohepatitis in male low-density lipoprotein receptor knockout mice. Gupte AA, Liu JZ, Ren Y, Minze LJ, Wiles JR, Collins AR, Lyon CJ, Pratico D, Finegold MJ, Wong ST, Webb P, Baxter JD, Moore DD, Hsueh WA Hepatology (Baltimore, Md.), 2010 (52), 2001 - 2011		Tso, Patrick	20938947	Published
Myeloid deletion of nuclear factor erythroid 2-related factor 2 increases atherosclerosis and liver injury. Collins AR, Gupte AA, Ji R, Ramirez MR, Minze LJ, Liu JZ, Arredondo M, Ren Y, Deng T, Wang J, Lyon CJ, Hsueh WA Arteriosclerosis, thrombosis, and vascular biology, 2012 (32), 2839 - 2846		Minze, Laurie	23023374	Published

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