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Experiment

Circulating glucose, insulin, and estradiol levels in Ldlr KO mice fed high fat
diet
Summary Data Summary
Investigator Villablanca, Amparo
Description Mice (5 weeks of age) are fed either a chow or high-fat diet (Harlan cat. no.
TD.88137) for 8 weeks. They have ad lib access to food and water. Animals at the
age of 13 weeks are fasted roughly 12 hours prior to euthanization by cardiac
puncture and exsanguination. Blood is drawn by cardiac puncture and collected in
serum separator tubes. Samples are spun and serum fraction is immediately drawn
off and frozen at -80C until use.
Status Completed
Public Release 1/10/2021
Animal Age Measured In: week(s) post-natal (w)
Flags has-data-flagSame StrainNeed Conditions
Data Analysis
TypeCount
Animals12
Experimental Conditions5
Catalog Items3
Curation Info (# flags)2
Phenotype Assays3
Phenotype Measurements36
Histology Images0
Publications0


Animals
Strain NameCommon NameFemalesMalesUnknown
C57BL/6J
6
0
0
C57BL/6J-Ldlr-/-
6
0
0

Animals/Strains
Experimental Factors
 Categorical Values
Name / AbbreviationDescription

Experimental Factor: Experimental Group

 Control
This animal belongs to the control group for the experiment.
 Experiment
This animal belongs to experimental group that is homozygous for gene manipulations.

Experimental Factor: Feeding Group

 Fasted
Animals are deprived of food but not water.

Experimental Factor: Mouse Diet

 Teklad - 08485
Harlan Teklad TD.08485 Low Fat Control Diet Purified Diet, Modification of TD.88137, Low Fat & No Added Cholesterol, Reduced Sucrose.
 Teklad - 88137
Harlan Teklad TD.88137 (Western Diet)

Experimental Factor: Tissue Source

 serum
serum
Experimental Factor Values


Phenotype Assays Add / Edit


No documents found.

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