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Experiment

Sable Energy Balance #22216 3/13/17
Summary Data Summary
Investigator Hemnes, Anna
Description Energy expenditure measured by indirect calorimetry in the Promethion by Sable
Systems. Activity, food intake and water intake are also continuously measured.
Measurements were taken for 5 consecutive days.
Running wheels were not present in the cage.
Mice were fed ad libitum.
Water: regular tap water, ad libitum.

Groups:
Male and Female WT(C57BL/6J) and Het (C57BL/6-Cav1tm1Mls/+/J
[C57BL/6-Cav1-/+/J]) mice.
Mice were fed a chow diet (LabDiet PicoLab Rodent Diet 5LOD) or a 60% kcal High
Fat Diet (F3282 BioServ).
Male WT Chow: n=4,
Male Het Chow: n=2,
Female WT Chow: n=2,
Female Het Chow: n=1
Male WT HF: n=3,
Male Het HF: n=0,
Female WT HF: n=3,
Female Het HF: n=0

Purpose of the experiment: This experiment will determine whether the groups of
mice have different energy expenditure and feeding behaviors.

Applicable Research Areas: Metabolism , Body weight regulation, energy
expenditure, feeding behavior.
Status Completed
Public Release 4/5/2019
Animal Age Measured In: day(s) post-natal (d)
Flags has-data-flagSame StrainAnimal Count
Data Analysis
TypeCount
Animals15
Experimental Conditions2
Catalog Items1
Curation Info (# flags)2
Phenotype Assays14
Phenotype Measurements210
Histology Images0
Publications0


Animals

Strain NameCommon NameFemalesMalesUnknown
C57BL/6J
5
6
0
C57BL/6-Cav1-/+
1
3
0


Experimental Factors
 Name / AbbreviationDescription

Experimental Factor: Experimental Group

 Control
This animal belongs to the control group for the experiment.
 Experiment
This animal belongs to experimental group that is homozygous for gene manipulations.

Experimental Factor: Mouse Diet

 BioServ F3282
Rodent Hig Fat Diet. Protein 20.%, Fat 35.5%, Fiber 0.0%, Ash 3.2%, Moisture <5.0%, Carbohydrate 36.3%. Ingredients: Lard, Casein, Dextrose, Sucrose, DL-Methionine, Choline Chloride, Vitamin Mix and Mineral Mix
 LabDiet PicoLab Rodent Diet 5LOD
Crude Protein 23%, crude fat 4.5%, crude fiber 6.0%. Ingredients: dehulled soybean meal, ground corn, dried beet pulp, fish meal, ground oats, dehydrated alfalfa meal, cane molasses, brewers dried yeast, wheat germ, whey, porcine animal fat...



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