MMPC :: Experiment

Experiment

Circulating insulin, lipid, ALT, and AST levels in B6 mice fed high fat diet and
treated with niclosamide

Summary

Data Summary

Investigator	Shengkan, Jin
Description	"Oral niclosamide ethanolamine (NEN) increases energy expenditure and lipid metabolism in mice. It is also efficacious in preventing and treating hepatic steatosis and insulin resistance induced by a high-fat diet. Moreover, it improves glycemic control and delays disease progression in db/db mice. Given the well-documented safety profile of NEN, it provides a potentially new and practical pharmacological approach for treating T2D. 5 week old male C57BL/6J mice were treated with high fat diet(HFD) for 21 weeks. MB1-47 and MB1-78 both derivatives of niclosamide ethanolamine were added to the diet at concentrations of 150mg and 300mg per kg of diet. After 5 hour fasting, mice were anesthetized with ketamine and xylazine to draw blood from heart with heparin-coated tube. Plasma was obtained by centrifugation at 1000g for 10-15min at 4? , and stored at -80? for further analysis." Applicable research area(s): Diabetes, Metabolism, Endocrine deficiency
Status	Completed
Public Release	8/8/2019
Animal Age	Measured In: week(s) post-natal (w)
Flags

Data Analysis

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Animals

Strain Name	Common Name	Females	Males	Unknown
C57BL/6	C57BL/6	0	21	0

Animals/Strains

Experimental Factors

	Categorical Values
	Name / Abbreviation	Description
	Experimental Factor: Experimental Group
	Control	This animal belongs to the control group for the experiment.
	Experiment	This animal belongs to experimental group that is homozygous for gene manipulations.
	Experimental Factor: Feeding Group
	Fasted	Animals are deprived of food but not water.
	Experimental Factor: Mouse Diet
	Research Diets - D12492	Rodent Diet with 34% Fat, 60% kcal from Fat
	Research Diets - D16121601	Rodent Diet with 60% kcal fat, 20% kcal carbohydrate, 20% kcal protein supplemented with 300 mg/kg MB1-47, a deriviative of niclosamide ethanolamine (NEN)
	Research Diets - D17041902	Rodent Diet with 60% kcal fat, 20% kcal carbohydrate, 20% kcal protein supplemented with 150 mg/kg MB1-78, a deriviative of niclosamide ethanolamine (NEN)
	Experimental Factor: Tissue Source
	plasma (sodium heparin)	plasma (sodium heparin)

Experimental Factor Values

Phenotype Assays Add / Edit

Name	Abbreviation
cholesterol, total	TC
triglycerides	TG
alanine aminotransferase	ALT
aspartate aminotransferase	AST
cholesterol, HDL, plasma	HDL-cholesterol
cholesterol, LDL and VLDL	LDL/VLDL

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