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Experiment

Liver lipid levels in Lepr KO mice treated with derivatives of niclosamide
Summary Data Summary
Investigator Shengkan, Jin
Description BKS.Cg-Dock7m +/+ Leprdb/J mice received daily oral gavage of vehicle or
1.0mg/kg or 2.5mg/kg body weight of WX53 or 2.5mg/kg or 5.0mg/kg body weight of
Y03 for 3 weeks. C57BL6/J mice with Research Diets-D12492 received daily oral
gavage of 1.0mg/kg or 2.5mg/kg or 5.0mg/kg body weight of WX53 or 2.5mg/kg or
5.0mg/kg body weight of Y03 for 3 weeks. 5 hour-fasted blood was collected
through tail vein bleeding at the end of the treatment. Plasma was obtained by
centrifugation at 1000g for 10-15min at 4? . Whole blood and plasma stored at
-80? for further analysis. WX53 and Y03 are from a group of mitochondrial
uncouplers that can stimulates oxygen consumption. Hypothesis: oral
administration of mitochondrial uncouplers could reduce blood glucose level and
improve insulin resistance. The objective of the study is to investigate the
effect of mitochondrial uncouplers on the treatment of type 2 diabetes.

Applicable research areas: Diabetes, Endocrine deficiency, Metabolism
Status Completed
Public Release 8/27/2021
Animal Age Measured In: week(s) post-natal (w)
Flags has-data-flagSame Strain
Data Analysis
TypeCount
Animals49
Experimental Conditions9
Catalog Items1
Curation Info (# flags)1
Phenotype Assays2
Phenotype Measurements98
Histology Images0
Publications0


Animals
Strain NameCommon NameFemalesMalesUnknown
C57BL/6J
0
24
0
BKS db
0
25
0

Experimental Factors
 Categorical Values
Name / AbbreviationDescription

Experimental Factor: Drug Administered

 Vehicle
Vehicle - Control
 WX53
WX53, a niclosamide derivative, in 2% DMSO, 10% solutol, 88% of 20% beta-cyclodextrin solution
 Y03
Y03, a niclosamide derivative, in 2% DMSO, 10% solutol, 88% of 20% beta-cyclodextrin solution.

Experimental Factor: Drug Delivery Route

 PO
The drug was delivered orally

Experimental Factor: Drug Dose (AU)

 Drug Dose
A numerical value indicating the dose of a drug administered. The unit of measure is indicated in the Drug Dose Units categorical value that accompanies this experimental condition.

Experimental Factor: Drug Dose Units

 mg/kg
milligrams per kilogram

Experimental Factor: Experimental Group

 Control
This animal belongs to the control group for the experiment.
 Experiment
This animal belongs to experimental group that is homozygous for gene manipulations.

Experimental Factor: Fast Duration (hr)

 fast duration
Length of time the animal was fasted

Experimental Factor: Feeding Group

 Fasted
Animals are deprived of food but not water.

Experimental Factor: Mouse Diet

 LabDiet 5001
crude protein (23.0%), crude fat (4.5%), crude fiber (6.0%), Ash (< 8.0%), minerals (2.5%)
 Research Diets - D12492
Rodent Diet with 34% Fat, 60% kcal from Fat

Experimental Factor: Tissue Source

 plasma (EDTA)
plasma (EDTA)


Phenotype Assays Add / Edit


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