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Experiment

Effect of Dinitrophenol (DNP) and mitochondrial uncouplers on Energy
expenditure.
Summary Data Summary
Investigator Sinick, Jonah
Description The objective of this study is to determine if a single 5, 10 or 20mg/kg IP dose
of 2-dinitrophenol (DNP) or mitochondrial uncoupler (BAM15 or Niclosamide) are
sufficient to elicit a change in murine energy expenditure. Male c57BL6/J ~9
weeks of age are acclimate for 1-2 week on std. vivarium chow (Teklad 2918)
before undergoing CLAMS. After acclimation to CLAMS, mice are injected with
saline on day 1 and compound of interest on day 2 of energy expenditure
analysis. Upon completion of CLAMS, body composition is assessed by DEXA.
Status Completed
Public Release 12/15/2022
Animal Age Measured In: week(s) post-natal (w)
Flags has-data-flagSame StrainAnimal Count
Data Analysis
TypeCount
Animals32
Experimental Conditions6
Catalog Items5
Curation Info (# flags)2
Phenotype Assays31
Phenotype Measurements1984
Histology Images0
Publications0


Animals
Strain NameCommon NameFemalesMalesUnknown
C57BL/6J
0
32
0

Animals/Strains
Experimental Factors
 Categorical Values
Name / AbbreviationDescription

Experimental Factor: Drug Administered

 2,4-Dinitrophenol
2,4-Dinitrophenol (DNP) is a precursor to other chemicals and is biochemically active, inhibiting adenosine triphosphate (ATP) production in cells with mitochondria.
 BAM15
BAM15: N5,N6-bis(2-fluorophenyl)-[1,2,5]oxadiazolo[3,4-b]pyrazine-5,6-diamine, a novel mitochondrial protonophore uncoupler capable of protecting mammals from acute renal ischemic-reperfusion injury and cold-induced microtubule damage
 Niclosamide
Niclosamide: inhibits glucose uptake and oxidative phosphorylation
 Vehicle
Vehicle - Control

Experimental Factor: Drug Delivery Route

 IP
The drug was delivered intraperitoneal.

Experimental Factor: Drug Dose Units

 mg/kg
milligrams per kilogram

Experimental Factor: Experimental Group

 Control
This animal belongs to the control group for the experiment.
 Experiment
This animal belongs to experimental group that is homozygous for gene manipulations.

Experimental Factor: Mouse Diet

 Teklad - 2918
Harlan-Teklad 2918 (Irradiated) 18.6% protein (Calories : 24% protein, 18% Fat, 58% Carbohydrate) Ingredients—Ground wheat, ground corn, wheat middlings, dehulled soybean meal, corn gluten meal, soybean oil, calcium carbonate, brewers dried yeast,...
Experimental Factor Values


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