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Experiment

Lipid droplet and mitochondrial analysis in aged MURF/1 KO Mice
Summary Data Summary
Investigator Bodine, Sue
Description The investigator will provide frozen gastronemus from 24m wt and Murk1 KO
animals. Lipid content/droplets abundance in muscle sections will be analyzed
using BIDIPY staining and mitochondrial content by immunohistochemistry
utilizing a mitochondrial specific antibody. In addition we have been requested
to treat cultured C2C12 cells differenteatedinto myotubes with lipids and
measure lipid loading with BODIPY staining.

Applicable research area(s): Obesity
Status Completed
Public Release 2/5/2017
Animal Age Measured In: month(s) post-natal (m)
Data Analysis
TypeCount
Animals2
Experimental Conditions1
Catalog Items1
Curation Info (# flags)0
Phenotype Assays1
Phenotype Measurements2
Histology Images4
Publications0


Animals

Strain NameCommon NameFemalesMalesUnknown
C57BL/6J
0
1
0
B6;129S1-Trim63-/-
0
1
0


Experimental Factors
 Name / AbbreviationDescription

Experimental Factor: Experimental Group

 Control
This animal belongs to the control group for the experiment.
 Experiment
This animal belongs to experimental group that is homozygous for gene manipulations.


Phenotype Assays Add / Edit


Histology Images
CaptionStrainAnatomical Site
Lipid droplet formation in muscle of aged (24 month) wild type mouseC57BL/6Jgastrocnemius muscle
Assessment of mitochondrial abundance and localization in muscle of aged (24 month) wild type mouseC57BL/6Jgastrocnemius muscle
Assessment of mitochondrial abundance and localization in muscle of aged (24 month) MuRF1 KO mouseB6;129S1-Trim63tm1Glasgastrocnemius muscle
Assessment of lipid droplet formation in muscle of aged (24 month) MuRF1 KO miceB6;129S1-Trim63tm1Glasgastrocnemius muscle



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