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Experiment

Lipid droplet and mitochondrial analysis in aged MURF/1 KO Mice
Summary Data Summary
Investigator Bodine, Sue
Description The investigator will provide frozen gastronemus from 24m wt and Murk1 KO
animals. Lipid content/droplets abundance in muscle sections will be analyzed
using BIDIPY staining and mitochondrial content by immunohistochemistry
utilizing a mitochondrial specific antibody. In addition we have been requested
to treat cultured C2C12 cells differenteatedinto myotubes with lipids and
measure lipid loading with BODIPY staining.

Applicable research area(s): Obesity
Status Completed
Public Release 2/5/2017
Animal Age Measured In: month(s) post-natal (m)
Data Analysis
TypeCount
Animals2
Experimental Conditions1
Catalog Items1
Curation Info (# flags)0
Phenotype Assays1
Phenotype Measurements2
Histology Images4
Publications0


Animals

Strain NameCommon NameFemalesMalesUnknown
C57BL/6J
0
1
0
B6;129S1-Trim63-/-
0
1
0

Experimental Factors
 Categorical Values
Name / AbbreviationDescription

Experimental Factor: Experimental Group

 Control
This animal belongs to the control group for the experiment.
 Experiment
This animal belongs to experimental group that is homozygous for gene manipulations.
Experimental Factor (Units)
Experimental Group


Phenotype Assays Add / Edit


Histology Images
CaptionStrainAnatomical Site
Lipid droplet formation in muscle of aged (24 month) wild type mouseC57BL/6Jgastrocnemius muscle
Assessment of mitochondrial abundance and localization in muscle of aged (24 month) wild type mouseC57BL/6Jgastrocnemius muscle
Assessment of mitochondrial abundance and localization in muscle of aged (24 month) MuRF1 KO mouseB6;129S1-Trim63tm1Glasgastrocnemius muscle
Assessment of lipid droplet formation in muscle of aged (24 month) MuRF1 KO miceB6;129S1-Trim63tm1Glasgastrocnemius muscle



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