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Lipid droplet and mitochondrial analysis in aged MURF/1 KO Mice
The investigator will provide frozen gastronemus from 24m wt and Murk1 KO
animals. Lipid content/droplets abundance in muscle sections will be analyzed
using BIDIPY staining and mitochondrial content by immunohistochemistry
utilizing a mitochondrial specific antibody. In addition we have been requested
to treat cultured C2C12 cells differenteatedinto myotubes with lipids and
measure lipid loading with BODIPY staining.
Applicable research area(s): Obesity
month(s) post-natal (m)
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Curation Info (# flags)
Name / Abbreviation
Experimental Factor: Experimental Group
This animal belongs to the control group for the experiment.
This animal belongs to experimental group that is homozygous for gene manipulations.
Experimental Factor (Units)
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Lipid droplet formation in muscle of aged (24 month) wild type mouse
Assessment of mitochondrial abundance and localization in muscle of aged (24 month) wild type mouse
Assessment of mitochondrial abundance and localization in muscle of aged (24 month) MuRF1 KO mouse
Assessment of lipid droplet formation in muscle of aged (24 month) MuRF1 KO mice
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Financial support for this work was provided by the NIDDK Mouse Metabolic Phenotyping Centers (National MMPC, RRID:SCR_008997,
) under the MICROMouse Program, grants DK076169.
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