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Publication
Genetic dissection in a mouse model reveals interactions between carotenoids and
lipid metabolism.
Authors Palczewski G, Widjaja-Adhi MA, Amengual J, Golczak M, von Lintig J
Submitted By Submitted Externally on 10/26/2016
Status Published
Journal Journal of lipid research
Year 2016
Date Published 9/1/2016
Volume : Pages 57 : 1684 - 95
PubMed Reference 27389691
Abstract Carotenoids affect a rich variety of physiological functions in nature and are
beneficial for human health. However, knowledge about their biological action
and the consequences of their dietary accumulation in mammals is limited.
Progress in this research field is limited by the expeditious metabolism of
carotenoids in rodents and the confounding production of apocarotenoid signaling
molecules. Herein, we established a mouse model lacking the enzymes responsible
for carotenoid catabolism and apocarotenoid production, fed on either a
ß-carotene- or a zeaxanthin-enriched diet. Applying a genome wide microarray
analysis, we assessed the effects of the parent carotenoids on the liver
transcriptome. Our analysis documented changes in pathways for liver lipid
metabolism and mitochondrial respiration. We biochemically defined these
effects, and observed that ß-carotene accumulation resulted in an elevation of
liver triglycerides and liver cholesterol, while zeaxanthin accumulation
increased serum cholesterol levels. We further show that carotenoids were
predominantly transported within HDL particles in the serum of mice. Finally, we
provide evidence that carotenoid accumulation influenced whole-body respiration
and energy expenditure. Thus, we observed that accumulation of parent
carotenoids interacts with lipid metabolism and that structurally related
carotenoids display distinct biological functions in mammals.




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