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Strain
FVB.129S2(B6)-Hmox1tm1Poss/J

Summary Data Summary
Official Name FVB.129S2(B6)-Hmox1tm1Poss/J
Common Name FVB.129S2(B6)-Hmox1tm1Poss/J
Description A 3.7 kb fragment encompassing exons 3, 4, and a portion of
5 was replaced with a neomycin selection cassette. The
deleted region consisted of approximately 85% of the coding
region (226 residues). A low level of aberrantly spliced
transcript was identified in homozygous mutant mice by
Northern blot analysis of total splenic RNA.
Development Status Phenotyping ongoing
Creation Method knockout
Phenotype Description Mice that are homozygous for this targeted mutation are
slightly smaller in size than wildtype littermates and
exhibit poor grooming and hypoactivity. As early as 20 weeks
of age, homozygotes develop anemia with diminished serum
iron and increased serum ferritin. Histological analysis
reveals iron accumulation in kidney and liver. Elevated
oxidized proteins and lipid peroxidation develop in the
liver and kidney. Homozygotes develop progressive chronic
inflammatorydisease, including enlarged spleen and lymph
nodes, inflammatory infiltrates, glomerulonephritis,
fibrosis. Homozygous male mice have smaller testis than
wildtype controls. Homozygotes occur at a lower than
expected frequency, or are not produced, from heterozygous
crosses and have decreased postnatal survival. An almost
undetectable abnormal gene product (mRNA) is detected by
Northern blot analysis of total splenic RNA. This mutant
mouse strain may be useful in studies of hemochromatosis,
inflammation and iron metabolism.
TypeCount
Investigators 1
Genomics - Modifications 1
Experiments 1


Investigators
NameInstitution
Gerald I. ShulmanYale University


Genomic Information
GeneAllele 1Allele 2Protocol
Hmox1knockoutknockoutNot Specified


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