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Strain
C57BL/6-Tg(pTRE-E4orf1)

Summary Data Summary
Official Name C57BL/6-Tg(pTRE-E4orf1)
Common Name C57BL/6-Tg(pTRE-E4orf1)
Description To generate a doxycycline (Dox)-inducible mouse model of
E4orf1 overexpression, E4orf1 cDNA was amplified and
engineered into pENTR vector (Invitrogen) and verified by
sequencing from both ends (Genewiz). To facilitate cloning,
we engineered Gateway components (Invitrogen) into pTRE
vector (Clontech). A rabbit ß-globin 3'UTR was included to
stabilize the transcript and enhance the translation. This
pTRE-Gateway-3'UTR vector was used for one-step cloning of
E4orf1 into pTRE construct by recombination. The expression
of E4orf1 is controlled by 7 tandem repeats of tetracycline
responsive elements in front of a minimum CMV promoter. The
pTRE-E4orf1 DNA was linearized by Xmn I, Ale I, Cla I and
Ahd I digestion. A 5.2-kb fragment was purified by Elutip-D
and injected into embryos of pure C57/Bl6 background by the
transgenic core facility at UTSW. Transgene-positive
TRE-E4orf1 offspring were genotyped using PCR with the
primer set: 5'-GGCATACTAACCCAGTCCGATG and
5'-AATCACTCTCTCCAGCAGCAGG.
Development Status Phenotyping ongoing
Creation Method transgenic
Phenotype Description We have generated a novel mouse model that allows us to
constitutively activate an adenoviral protein, E4orf1, in an
inducible fashion, specifically in the adipose tissue. In
transgenic mice, the expression of E4orf1 improves glucose
disposal and prevents liver fat accumulation independent of
insulin, as compared to wild type controls.
TypeCount
Investigators 1
Experiments 1


Investigators
NameInstitution
Vijay HegdeTexas Tech University


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