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Strain
C57BL/6-Calcrcre

Summary Data Summary
Official Name C57BL/6-Calcrcre
Common Name Calcrcre
Description To generate Calcrcre mice, a selection cassette containing
the porcine teschoviral 2A cleavage sequence linked to Cre
recombinase and a Frt-flanked neomycin resistance gene was
targeted to replace the stop codon of the Calcr gene in a
bacterial artificial chromosome (RP24-193M22; Children’s
Hospital Oakland Research Institute). A targeting plasmid
containing the Cre-containing selection cassette and ~4-kb
genomic sequence upstream and downstream of the Calcr stop
codon was isolated and used for embryonic stem cell
targeting by the University of Michigan Transgenic Core.
Correctly targeted clones were identified by loss of native
allele quantitative polymerase chain reaction (PCR) from
embryonic stem cell clone DNA. Chimeric animals generated
from blastocyst implantation were then bred for germline
transmission of the Calcrcre allele. Flp-deleter mice were
then used to remove the neomycin selection cassette.
Genotyping was by allele-specific PCR.
Development Status Phenotyping ongoing
Creation Method knockin
TypeCount
Investigators 1
Genomics - Modifications 1
Experiments 12


Investigators
NameInstitution
Martin MyersUniversity of Michigan-Ann Arbor


Genomic Information
GeneAllele 1Allele 2Protocol
Calcrknockin (Cre)knockin (Cre)Not Specified


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