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Strain
B6.Cg-Cftrtm1.1Cwr Tg(Ucp1-cre)1Evdr/J

Summary Data Summary
Official Name B6.Cg-Cftrtm1.1Cwr Tg(Ucp1-cre)1Evdr/J
Common Name CFTR-UCP1 CRE
Description Cftr: Germ line, cre mediated recombination was
used to remove exon 11 (exon 10 in legacy nomenclature
numbering). Absence of full-length transcript expression was
confirmed by RT-PCR on lung and intestine extracts.
Expression of a smaller than normal transcript is observed.

Tg(Ucp1-cre)1Evd: The C57BL/6 mouse bacterial artificial
chromosome (BAC) 148M1 containing the entire Ucp1 gene, and
other genes, was modified by inserting sequence encoding cre
recombinase, into the translation initiation site into exon
1 of the Ucp1 gene. An FRT site flanked KAN cassette and a
loxP site were removed. DNA from the modified BAC clone was
used as the source for the transgene, which was
microinjected into the pronucleus of FVB fertilized eggs. A
founder line was subsequently established.
Development Status Phenotyping ongoing
Creation Method transgenic
TypeCount
Investigators 1
Genomics - Modifications 1
Genomics - Transgenes 1
Experiments 2


Investigators
NameInstitution
John YoonUniversity of California Davis


Genomic Information
GeneAllele 1Allele 2Protocol
Cftrknockin (lox)knockin (lox)Not Specified
EnhancerPromoterGeneCopies
Not SpecifiedUcp1CRENot Specified


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