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Strain
C57BL/6-Snord116tm1.1Uta/+

Summary Data Summary
Official Name C57BL/6-Snord116tm1.1Uta/+
Common Name paternal Snord116del -/+
Description Heterozygous deletion male mice were bred with C57BL/6 WT
females to generate paternal deletion Snord116del+/- and WT
littermates.

Snord116 del: Two individual targeting vectors were used to
place a loxP site (and an Frt1-flanked PGK-neo cassette)
upstream, and a loxP site (and an Frt5-flanked puromycin
resistance/TK cassette) downstream of the Snord116 cluster.
The upstream targeting vector was transfected into
C57BL/6-derived Bruce-4 embryonic stem (ES) cells, and
correctly targeted ES cells were next transfected with the
downstream targeting vector. Doubly targeted ES cells were
then transiently transfected with a cre expressing plasmid.
The resulting 1-loxP ES cells (with the Snord116 cluster
deleted and a single loxP site remaining) were injected into
recipient blastocysts.
Development Status Not specified/Other
Creation Method knockout
Maternal Strain C57BL/6
Paternal Strain B6(Cg)-Snord116tm1.1Uta/J
TypeCount
Investigators 1
Genomics - Modifications 1
Experiments 2
Publications 1


Investigators
NameInstitution
Dag YasuiUniversity of California Davis


Genomic Information
GeneAllele 1Allele 2Protocol
Snord116knockoutwildNot Specified






PublicationAltmetricsSubmitted ByPubMed IDStatus
A Prader-Willi locus lncRNA cloud modulates diurnal genes and energy expenditure.
Powell WT, Coulson RL, Crary FK, Wong SS, Ach RA, Tsang P, Alice Yamada N, Yasui DH, Lasalle JM
Human molecular genetics, 2013 (22), 4318 - 4328
Submitted Externally
23771028
Published

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